A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes

Marta Bottagisio, A. F. Pellegata, F. Boschetti, M. Ferroni, M. Moretti, Arianna Barbara Lovati

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tendon ruptures and/or large losses remain to be a great clinical challenge and often require full replacement of the damaged tissue. The use of auto- and allografts or engineered scaffolds is an established approach to restore severe tendon injuries. However, these grafts are commonly related to scarce biocompatibility, site morbidity, chronic inflammation and poor biomechanical properties. Recently, the decellularisation techniques of allo- or xenografts using specific detergents have been studied and have been found to generate biocompatible substitutes that resemble the native tissue. This study aims to identify a novel decellularisation protocol for large equine tendons that would produce an extracellular matrix scaffold suitable for the regeneration of injured tendons in humans. Specifically, equine tendons were treated either with tri (n-butyl) phosphate alone, or associated to multiple concentrations of peracetic acid (1, 3 and 5 %), which has never before been tested in vitro. Samples were then analysed by histology and with biochemical, biomechanical, and cytotoxicity tests. The best decellularisation protocol, resulting from these examinations, was selected and the chosen scaffold was re-seeded with murine fibroblasts. Resulting grafts were tested for cell viability, histologic analysis, DNA and collagen content. The results identified 1 % tri (n-butyl) phosphate combined with 3 % peracetic acid as the most suitable decellularised matrix in terms of biochemical and biomechanical properties. Moreover, the non-cytotoxic nature of the decellularised matrix allowed for good fibroblast reseeding, thus demonstrating a biocompatible matrix that will be suitable for tendon tissue engineering and hopefully as substitutes in severe tendon damages.

Original languageEnglish
Pages (from-to)58-73
Number of pages16
JournalEuropean Cells and Materials
Volume32
Publication statusPublished - Jul 1 2016

Fingerprint

Tendons
Horses
Peracetic Acid
Scaffolds
Fibroblasts
Allografts
Grafts
Phosphates
Tendon Injuries
Transplants
Tissue
Autografts
Tissue Engineering
Histology
Acids
Heterografts
Detergents
Cytotoxicity
Extracellular Matrix
Regeneration

Keywords

  • Biomaterials
  • Biomechanics
  • Cell adhesion
  • Decellularisation
  • Equine tendon
  • Xenograft

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials

Cite this

Bottagisio, M., Pellegata, A. F., Boschetti, F., Ferroni, M., Moretti, M., & Lovati, A. B. (2016). A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes. European Cells and Materials, 32, 58-73.

A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes. / Bottagisio, Marta; Pellegata, A. F.; Boschetti, F.; Ferroni, M.; Moretti, M.; Lovati, Arianna Barbara.

In: European Cells and Materials, Vol. 32, 01.07.2016, p. 58-73.

Research output: Contribution to journalArticle

Bottagisio, M, Pellegata, AF, Boschetti, F, Ferroni, M, Moretti, M & Lovati, AB 2016, 'A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes', European Cells and Materials, vol. 32, pp. 58-73.
Bottagisio, Marta ; Pellegata, A. F. ; Boschetti, F. ; Ferroni, M. ; Moretti, M. ; Lovati, Arianna Barbara. / A new strategy for the decellularisation of large equine tendons as biocompatible tendon substitutes. In: European Cells and Materials. 2016 ; Vol. 32. pp. 58-73.
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