A new subtraction technique for molecular cloning of rare antiviral antibody specificities from phage display libraries

R. Burioni, P. Plaisant, F. Bugli, L. Solforosi, V. Delli Carri, P. E. Varaldo, G. Fadda

Research output: Contribution to journalArticle

Abstract

The preparation of random combinatorial libraries exposed on the surface of phage provides a route for the selection of diverse high affinity human monoclonal antibodies. However, in particular settings, the isolation of genes coding for a rare antibody can be elusive because some epitopes are predominant and because, in the case of impure antigens, the protein or any compound of interest can be present in relatively minimal amount. In this paper, we describe the successful utilization of a new strategy of 'preadsorption' panning that allowed us to clone a rare human monoclonal antibody fragment and to access a different antibody repertoire. The procedure is easy, fast, inexpensive, can be used together with other panning techniques and can be particularly useful in cloning antibodies against rare or unknown determinants.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalResearch in Virology
Volume149
Issue number5
DOIs
Publication statusPublished - Sep 1998

Fingerprint

Subtraction Technique
Antibody Specificity
Molecular Cloning
Bacteriophages
Libraries
Antiviral Agents
Antibodies
Monoclonal Antibodies
Immunoglobulin Fragments
Organism Cloning
Epitopes
Clone Cells
Antigens
Genes
Proteins

Keywords

  • Combinatorial library
  • Human monoclonal antibodies
  • Phage display

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A new subtraction technique for molecular cloning of rare antiviral antibody specificities from phage display libraries. / Burioni, R.; Plaisant, P.; Bugli, F.; Solforosi, L.; Delli Carri, V.; Varaldo, P. E.; Fadda, G.

In: Research in Virology, Vol. 149, No. 5, 09.1998, p. 327-330.

Research output: Contribution to journalArticle

Burioni, R. ; Plaisant, P. ; Bugli, F. ; Solforosi, L. ; Delli Carri, V. ; Varaldo, P. E. ; Fadda, G. / A new subtraction technique for molecular cloning of rare antiviral antibody specificities from phage display libraries. In: Research in Virology. 1998 ; Vol. 149, No. 5. pp. 327-330.
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