A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

Mara Canovi, Jacopo Lucchetti, Matteo Stravalaci, Sonia Valentino, Barbara Bottazzi, Mario Salmona, Antonio Bastone, Marco Gobbi

Research output: Contribution to journalArticle

Abstract

A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5-1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15-1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is

Original languageEnglish
Pages (from-to)10864-10875
Number of pages12
JournalSensors (Switzerland)
Volume14
Issue number6
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Medicine(all)

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