A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

Mara Canovi; Jacopo Lucchetti; Matteo Stravalaci; Sonia Valentino; Barbara Bottazzi; Mario Salmona; Antonio Bastone; Marco Gobbi

doi:10.3390/s140610864

A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

Mara Canovi, Jacopo Lucchetti, Matteo Stravalaci, Sonia Valentino, Barbara Bottazzi, Mario Salmona, Antonio Bastone, Marco Gobbi

Research output: Contribution to journal › Article

Abstract

A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5-1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15-1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is

Original language	English
Pages (from-to)	10864-10875
Number of pages	12
Journal	Sensors (Switzerland)
Volume	14
Issue number	6
DOIs	https://doi.org/10.3390/s140610864
Publication status	Published - 2014

ASJC Scopus subject areas

Medicine(all)

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10.3390/s140610864

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Canovi, M., Lucchetti, J., Stravalaci, M., Valentino, S., Bottazzi, B., Salmona, M., Bastone, A., & Gobbi, M. (2014). A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma. Sensors (Switzerland), 14(6), 10864-10875. https://doi.org/10.3390/s140610864

A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma. / Canovi, Mara; Lucchetti, Jacopo; Stravalaci, Matteo; Valentino, Sonia ; Bottazzi, Barbara ; Salmona, Mario; Bastone, Antonio; Gobbi, Marco.

In: Sensors (Switzerland), Vol. 14, No. 6, 2014, p. 10864-10875.

Research output: Contribution to journal › Article

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