A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

Mara Canovi; Jacopo Lucchetti; Matteo Stravalaci; Sonia Valentino; Barbara Bottazzi; Mario Salmona; Antonio Bastone; Marco Gobbi

doi:10.3390/s140610864

A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

Mara Canovi, Jacopo Lucchetti, Matteo Stravalaci, Sonia Valentino, Barbara Bottazzi, Mario Salmona, Antonio Bastone, Marco Gobbi

Research output: Contribution to journal › Article › peer-review

Abstract

A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5-1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15-1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is

Original language	English
Pages (from-to)	10864-10875
Number of pages	12
Journal	Sensors (Switzerland)
Volume	14
Issue number	6
DOIs	https://doi.org/10.3390/s140610864
Publication status	Published - 2014

ASJC Scopus subject areas

Medicine(all)

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10.3390/s140610864

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title = "A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma",

abstract = "A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5-1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15-1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is ",

author = "Mara Canovi and Jacopo Lucchetti and Matteo Stravalaci and Sonia Valentino and Barbara Bottazzi and Mario Salmona and Antonio Bastone and Marco Gobbi",

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T1 - A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

AU - Canovi, Mara

AU - Lucchetti, Jacopo

AU - Stravalaci, Matteo

AU - Valentino, Sonia

AU - Bottazzi, Barbara

AU - Salmona, Mario

AU - Bastone, Antonio

AU - Gobbi, Marco

PY - 2014

Y1 - 2014

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AB - A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5-1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15-1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is

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A new surface plasmon resonance-based immunoassay for rapid, reproducible and sensitive quantification of pentraxin-3 in human plasma

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