A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( 201Tl), C-14-deoxyglucose ( 14C-DG) and Scandium-46 ( 46Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i 1) and after (i 2) 201Tl decay (73 hours half-life) to insignificant level. Net 201Tl distribution image was obtained by digital subtraction of i 2 from i 1. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.
|Title of host publication||Journal of Nuclear Medicine and Allied Sciences|
|Number of pages||7|
|Publication status||Published - 1981|
ASJC Scopus subject areas