A new technique for multitracer macroscopic autoradiography

A. L'Abbate; P. Camici; G. Pelosi; M. G. Trivella; G. Valli; M. Marzilli; D. Levantesi; L. Taddei

A new technique for multitracer macroscopic autoradiography

A. L'Abbate, P. Camici, G. Pelosi, M. G. Trivella, G. Valli, M. Marzilli, D. Levantesi, L. Taddei

IRCCS Ospedale San Raffaele

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( ²⁰¹Tl), C-14-deoxyglucose ( ¹⁴C-DG) and Scandium-46 ( ⁴⁶Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i ₁) and after (i ₂) ²⁰¹Tl decay (73 hours half-life) to insignificant level. Net ²⁰¹Tl distribution image was obtained by digital subtraction of i ₂ from i ₁. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.

Original language	English
Title of host publication	Journal of Nuclear Medicine and Allied Sciences
Pages	27-33
Number of pages	7
Volume	25
Edition	1-2
Publication status	Published - 1981

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "A new technique for multitracer macroscopic autoradiography",

abstract = "A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( 201Tl), C-14-deoxyglucose ( 14C-DG) and Scandium-46 ( 46Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i 1) and after (i 2) 201Tl decay (73 hours half-life) to insignificant level. Net 201Tl distribution image was obtained by digital subtraction of i 2 from i 1. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.",

author = "A. L'Abbate and P. Camici and G. Pelosi and Trivella, {M. G.} and G. Valli and M. Marzilli and D. Levantesi and L. Taddei",

year = "1981",

language = "English",

volume = "25",

pages = "27--33",

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edition = "1-2",

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TY - CHAP

T1 - A new technique for multitracer macroscopic autoradiography

AU - L'Abbate, A.

AU - Camici, P.

AU - Pelosi, G.

AU - Trivella, M. G.

AU - Valli, G.

AU - Marzilli, M.

AU - Levantesi, D.

AU - Taddei, L.

PY - 1981

Y1 - 1981

N2 - A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( 201Tl), C-14-deoxyglucose ( 14C-DG) and Scandium-46 ( 46Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i 1) and after (i 2) 201Tl decay (73 hours half-life) to insignificant level. Net 201Tl distribution image was obtained by digital subtraction of i 2 from i 1. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.

AB - A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( 201Tl), C-14-deoxyglucose ( 14C-DG) and Scandium-46 ( 46Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i 1) and after (i 2) 201Tl decay (73 hours half-life) to insignificant level. Net 201Tl distribution image was obtained by digital subtraction of i 2 from i 1. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.

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M3 - Chapter

C2 - 7252593

AN - SCOPUS:0019870820

VL - 25

SP - 27

EP - 33

BT - Journal of Nuclear Medicine and Allied Sciences

ER -

A new technique for multitracer macroscopic autoradiography

Abstract

ASJC Scopus subject areas

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