A new technique for multitracer macroscopic autoradiography

A. L'Abbate, P. Camici, G. Pelosi, M. G. Trivella, G. Valli, M. Marzilli, D. Levantesi, L. Taddei

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A new technique for multitracer autoradiography is described. Identification of different isotopes was made possible by using: 1) diffusible and particulate tracers; 2) different half-life isotopes; 3) repeated expositions of tissue microslices; 4) computer processing of the autoradiographs. The technique was applied to the study of regional myocardial perfusion and metabolism in the dog's heart. Three different tracers were simultaneously used: Thallium-201 ( 201Tl), C-14-deoxyglucose ( 14C-DG) and Scandium-46 ( 46Sc) carbonized microscopheres 15±2 micron in diameter. Transverse slices of the entire heart, 40 micron thick, were exposed for equal periods, before (i 1) and after (i 2) 201Tl decay (73 hours half-life) to insignificant level. Net 201Tl distribution image was obtained by digital subtraction of i 2 from i 1. Microsphere distribution for the assessment of regional coronary blood flow was obtained following identification by computer of the particles in adjacent autoradiographs and their integrated mapping.

Original languageEnglish
Title of host publicationJournal of Nuclear Medicine and Allied Sciences
Pages27-33
Number of pages7
Volume25
Edition1-2
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Medicine(all)

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