A new 'two step' procedure for 4.5 log depletion of T and B cells in allogeneic transplantation and of neoplastic cells in autologous transplantation

F. Bertolini, T. Thomas, M. Battaglia, N. Gibelli, P. Pedrazzoli, G. Robustelli Della Cuna

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To evaluate a new 'two step' method for purging T, B and neoplastic cells from hematopoietic progenitor cells (PC), PCs were collected by apheresis and some suspensions were deliberately contaminated with 2-5% breast cancer cells. PCs were first processed through CellPro columns for positive selection of cells that express CD34. After this first step, the mean CD34+ cell recovery was 68 ± 12%, and CD34+ cell purity was 61 ± 11%; CD3+ and neoplastic cell depletion were 2.1 ± 0.4 and 1.9 ± 0.4 logs, respectively. Cells were further processed through the StemSep device for direct depletion of T and B cells or of breast cancer cells. After the first and the second step, overall CD34+ cell recovery was 50 ± 7%, T and B cell removal was 4.7 ± 0.4 log and neoplastic cell purging was 4.4 ± 0.3 log, ie significantly superior to methods described in the past.

Original languageEnglish
Pages (from-to)615-619
Number of pages5
JournalBone Marrow Transplantation
Volume19
Issue number6
Publication statusPublished - Mar 2 1997

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Autologous Transplantation
Homologous Transplantation
B-Lymphocytes
T-Lymphocytes
Breast Neoplasms
Blood Component Removal
Hematopoietic Stem Cells
Suspensions
Equipment and Supplies

Keywords

  • Processing
  • Progenitor cell
  • Purging
  • Stem cell
  • Transplantation

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

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title = "A new 'two step' procedure for 4.5 log depletion of T and B cells in allogeneic transplantation and of neoplastic cells in autologous transplantation",
abstract = "To evaluate a new 'two step' method for purging T, B and neoplastic cells from hematopoietic progenitor cells (PC), PCs were collected by apheresis and some suspensions were deliberately contaminated with 2-5{\%} breast cancer cells. PCs were first processed through CellPro columns for positive selection of cells that express CD34. After this first step, the mean CD34+ cell recovery was 68 ± 12{\%}, and CD34+ cell purity was 61 ± 11{\%}; CD3+ and neoplastic cell depletion were 2.1 ± 0.4 and 1.9 ± 0.4 logs, respectively. Cells were further processed through the StemSep device for direct depletion of T and B cells or of breast cancer cells. After the first and the second step, overall CD34+ cell recovery was 50 ± 7{\%}, T and B cell removal was 4.7 ± 0.4 log and neoplastic cell purging was 4.4 ± 0.3 log, ie significantly superior to methods described in the past.",
keywords = "Processing, Progenitor cell, Purging, Stem cell, Transplantation",
author = "F. Bertolini and T. Thomas and M. Battaglia and N. Gibelli and P. Pedrazzoli and {Della Cuna}, {G. Robustelli}",
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AU - Bertolini, F.

AU - Thomas, T.

AU - Battaglia, M.

AU - Gibelli, N.

AU - Pedrazzoli, P.

AU - Della Cuna, G. Robustelli

PY - 1997/3/2

Y1 - 1997/3/2

N2 - To evaluate a new 'two step' method for purging T, B and neoplastic cells from hematopoietic progenitor cells (PC), PCs were collected by apheresis and some suspensions were deliberately contaminated with 2-5% breast cancer cells. PCs were first processed through CellPro columns for positive selection of cells that express CD34. After this first step, the mean CD34+ cell recovery was 68 ± 12%, and CD34+ cell purity was 61 ± 11%; CD3+ and neoplastic cell depletion were 2.1 ± 0.4 and 1.9 ± 0.4 logs, respectively. Cells were further processed through the StemSep device for direct depletion of T and B cells or of breast cancer cells. After the first and the second step, overall CD34+ cell recovery was 50 ± 7%, T and B cell removal was 4.7 ± 0.4 log and neoplastic cell purging was 4.4 ± 0.3 log, ie significantly superior to methods described in the past.

AB - To evaluate a new 'two step' method for purging T, B and neoplastic cells from hematopoietic progenitor cells (PC), PCs were collected by apheresis and some suspensions were deliberately contaminated with 2-5% breast cancer cells. PCs were first processed through CellPro columns for positive selection of cells that express CD34. After this first step, the mean CD34+ cell recovery was 68 ± 12%, and CD34+ cell purity was 61 ± 11%; CD3+ and neoplastic cell depletion were 2.1 ± 0.4 and 1.9 ± 0.4 logs, respectively. Cells were further processed through the StemSep device for direct depletion of T and B cells or of breast cancer cells. After the first and the second step, overall CD34+ cell recovery was 50 ± 7%, T and B cell removal was 4.7 ± 0.4 log and neoplastic cell purging was 4.4 ± 0.3 log, ie significantly superior to methods described in the past.

KW - Processing

KW - Progenitor cell

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KW - Stem cell

KW - Transplantation

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