A nonsynonymous TNFRSF11A variation increases NFκB activity and the severity of Paget's disease

Fernando Gianfrancesco, Domenico Rendina, Marco Di Stefano, Alessandra Mingione, Teresa Esposito, Daniela Merlotti, Salvatore Gallone, Sara Magliocca, Alice Goode, Daniela Formicola, Giovanna Morello, Robert Layfield, Annalisa Frattini, Gianpaolo De Filippo, Ranuccio Nuti, Mark Searle, Pasquale Strazzullo, Giancarlo Isaia, Giuseppe Mossetti, Luigi Gennari

Research output: Contribution to journalArticlepeer-review


Mutations in the SQSTM1 gene were identified as a common cause of Paget's disease of bone (PDB) but experimental evidence demonstrated that SQSTM1 mutation is not sufficient to induce PDB in vivo. Here, we identified two nonsynonymous single nucleotide polymorphisms (SNPs) (C421T, H141Y and T575C, V192A) in the TNFRSF11A gene, associated with PDB and with the severity of phenotype in a large population of 654 unrelated patients that were previously screened for SQSTM1 gene mutations. The largest effect was found for the T575C variant, yielding an odds ratio of 1.29 (p = 0.003), with the C allele as the risk allele. Moreover, an even more significant p-value (p = 0.0002) was observed in the subgroup of patients with SQSTM1 mutation, with an odds ratio of 1.71. Interestingly, patients with the C allele also showed an increased prevalence of polyostotic disease (68%, 53%, and 51% in patients with CC, CT, and TT genotypes, respectively; p = 0.01), as well as an increased number of affected skeletal sites (2.9, 2.5, and 2.0 in patients with CC, CT, and TT genotypes, respectively, p = 0.008). These differences increased when analyses were restricted to cases with SQSTM1 mutation. In human cell lines, cotrasfection with mutated SQSTM1 and TNFRSF11A A192 produced a level of activation of NFκB signaling greater than cotrasfection with wild-type SQSTM1 and TNFRSF11A V192, confirming genetics and clinical evidences. These results provide the first evidence that genetic variation within the OPG/RANK/RANKL system influences the severity of PBD in synergistic action with SQSTM1 gene mutations.

Original languageEnglish
Pages (from-to)443-452
Number of pages10
JournalJournal of Bone and Mineral Research
Issue number2
Publication statusPublished - 2012


  • V192A

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism


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