A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry

Adriana Basile, Pasquale Ferranti, Gabriella Pocsfalvi, Gianfranco Mamone, Nadia Miraglia, Simonetta Caira, Luigi Ambrosi, Leonardo Soleo, Nicola Sannolo, Antonio Malorni

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.

Original languageEnglish
Pages (from-to)527-540
Number of pages14
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number8
DOIs
Publication statusPublished - 2001

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Electrospray ionization
Liquid chromatography
Ionization
Mass spectrometry
Desorption
Hemoglobins
Lasers
Derivatives
Peptides
Metabolites
Isotopes
Dilution
Dosimetry
erythritol anhydride
Monitoring
1,3-butadiene

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry. / Basile, Adriana; Ferranti, Pasquale; Pocsfalvi, Gabriella; Mamone, Gianfranco; Miraglia, Nadia; Caira, Simonetta; Ambrosi, Luigi; Soleo, Leonardo; Sannolo, Nicola; Malorni, Antonio.

In: Rapid Communications in Mass Spectrometry, Vol. 15, No. 8, 2001, p. 527-540.

Research output: Contribution to journalArticle

Basile, Adriana ; Ferranti, Pasquale ; Pocsfalvi, Gabriella ; Mamone, Gianfranco ; Miraglia, Nadia ; Caira, Simonetta ; Ambrosi, Luigi ; Soleo, Leonardo ; Sannolo, Nicola ; Malorni, Antonio. / A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry. In: Rapid Communications in Mass Spectrometry. 2001 ; Vol. 15, No. 8. pp. 527-540.
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AU - Ferranti, Pasquale

AU - Pocsfalvi, Gabriella

AU - Mamone, Gianfranco

AU - Miraglia, Nadia

AU - Caira, Simonetta

AU - Ambrosi, Luigi

AU - Soleo, Leonardo

AU - Sannolo, Nicola

AU - Malorni, Antonio

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