A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry

Adriana Basile, Pasquale Ferranti, Gabriella Pocsfalvi, Gianfranco Mamone, Nadia Miraglia, Simonetta Caira, Luigi Ambrosi, Leonardo Soleo, Nicola Sannolo, Antonio Malorni

Research output: Contribution to journalArticle

Abstract

The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.

Original languageEnglish
Pages (from-to)527-540
Number of pages14
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number8
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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