Abstract
The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.
| Original language | English |
|---|---|
| Pages (from-to) | 527-540 |
| Number of pages | 14 |
| Journal | Rapid Communications in Mass Spectrometry |
| Volume | 15 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry. / Basile, Adriana; Ferranti, Pasquale; Pocsfalvi, Gabriella; Mamone, Gianfranco; Miraglia, Nadia; Caira, Simonetta; Ambrosi, Luigi; Soleo, Leonardo; Sannolo, Nicola; Malorni, Antonio.
In: Rapid Communications in Mass Spectrometry, Vol. 15, No. 8, 2001, p. 527-540.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry
AU - Basile, Adriana
AU - Ferranti, Pasquale
AU - Pocsfalvi, Gabriella
AU - Mamone, Gianfranco
AU - Miraglia, Nadia
AU - Caira, Simonetta
AU - Ambrosi, Luigi
AU - Soleo, Leonardo
AU - Sannolo, Nicola
AU - Malorni, Antonio
PY - 2001
Y1 - 2001
N2 - The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.
AB - The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.
UR - http://www.scopus.com/inward/record.url?scp=0035028770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035028770&partnerID=8YFLogxK
U2 - 10.1002/rcm.263
DO - 10.1002/rcm.263
M3 - Article
C2 - 11312501
AN - SCOPUS:0035028770
VL - 15
SP - 527
EP - 540
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
SN - 0951-4198
IS - 8
ER -