A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera

Massimo Marzorati, Alberto Alma, Luciano Sacchi, Massimo Pajoro, Simona Palermo, Lorenzo Brusetti, Noura Raddadi, Annalisa Balloi, Rosemarie Tedeschi, Emanuela Clementi, Silvia Corona, Fabio Quaglino, Piero Attilio Bianco, Tiziana Beninati, Claudio Bandi, Daniele Daffonchio

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Flavescence dorée (FD) is a grapevine disease that afflicts several wine production areas in Europe, from Portugal to Serbia. FD is caused by a bacterium, "Candidatus Phytoplasma vitis," which is spread throughout the vineyards by a leafhopper, Scaphoideus titanus (Cicadellidae). After collection of S. titanus specimens from FD-contaminated vineyards in three different areas in the Piedmont region of Italy, we performed a survey to characterize the bacterial microflora associated with this insect. Using length heterogeneity PCR with universal primers for bacteria we identified a major peak associated with almost all of the individuals examined (both males and females). Characterization by denaturing gradient gel electrophoresis confirmed the presence of a major band that, after sequencing, showed a 97 to 99% identity with Bacteroidetes symbionts of the "Candidatus Cardinium hertigii" group. In addition, electron microscopy of tissues of S. titanus fed for 3 months on phytoplasma-infected grapevine plants showed bacterial cells with the typical morphology of "Ca. Cardinium hertigii." This endosymbiont, tentatively designated ST1-C, was found in the cytoplasm of previtellogenic and vitellogenic ovarian cells, in the follicle cells, and in the fat body and salivary glands. In addition, cell morphologies resembling those of "Ca. Phytoplasma vitis" were detected in the midgut, and specific PCR assays indicated the presence of the phytoplasma in the gut, fat body and salivary glands. These results indicate that ST1-C and "Ca. Phytoplasma vitis" have a complex life cycle in the body of S. titanus and are colocalized in different organs and tissues.

Original languageEnglish
Pages (from-to)1467-1475
Number of pages9
JournalApplied and Environmental Microbiology
Volume72
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Scaphoideus titanus
Phytoplasma
phytoplasma
Insect Vectors
Bacteroidetes
insect vectors
Vitis
symbiont
Vitis vinifera
symbionts
insect
Fat Body
vineyard
fat body
Cicadellidae
salivary glands
Salivary Glands
vineyards
fat
Italy

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera. / Marzorati, Massimo; Alma, Alberto; Sacchi, Luciano; Pajoro, Massimo; Palermo, Simona; Brusetti, Lorenzo; Raddadi, Noura; Balloi, Annalisa; Tedeschi, Rosemarie; Clementi, Emanuela; Corona, Silvia; Quaglino, Fabio; Bianco, Piero Attilio; Beninati, Tiziana; Bandi, Claudio; Daffonchio, Daniele.

In: Applied and Environmental Microbiology, Vol. 72, No. 2, 02.2006, p. 1467-1475.

Research output: Contribution to journalArticle

Marzorati, M, Alma, A, Sacchi, L, Pajoro, M, Palermo, S, Brusetti, L, Raddadi, N, Balloi, A, Tedeschi, R, Clementi, E, Corona, S, Quaglino, F, Bianco, PA, Beninati, T, Bandi, C & Daffonchio, D 2006, 'A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera', Applied and Environmental Microbiology, vol. 72, no. 2, pp. 1467-1475. https://doi.org/10.1128/AEM.72.2.1467-1475.2006
Marzorati, Massimo ; Alma, Alberto ; Sacchi, Luciano ; Pajoro, Massimo ; Palermo, Simona ; Brusetti, Lorenzo ; Raddadi, Noura ; Balloi, Annalisa ; Tedeschi, Rosemarie ; Clementi, Emanuela ; Corona, Silvia ; Quaglino, Fabio ; Bianco, Piero Attilio ; Beninati, Tiziana ; Bandi, Claudio ; Daffonchio, Daniele. / A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 2. pp. 1467-1475.
@article{09d70a01d3d8482a9a3829e031a45bf1,
title = "A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dor{\'e}e in Vitis vinifera",
abstract = "Flavescence dor{\'e}e (FD) is a grapevine disease that afflicts several wine production areas in Europe, from Portugal to Serbia. FD is caused by a bacterium, {"}Candidatus Phytoplasma vitis,{"} which is spread throughout the vineyards by a leafhopper, Scaphoideus titanus (Cicadellidae). After collection of S. titanus specimens from FD-contaminated vineyards in three different areas in the Piedmont region of Italy, we performed a survey to characterize the bacterial microflora associated with this insect. Using length heterogeneity PCR with universal primers for bacteria we identified a major peak associated with almost all of the individuals examined (both males and females). Characterization by denaturing gradient gel electrophoresis confirmed the presence of a major band that, after sequencing, showed a 97 to 99{\%} identity with Bacteroidetes symbionts of the {"}Candidatus Cardinium hertigii{"} group. In addition, electron microscopy of tissues of S. titanus fed for 3 months on phytoplasma-infected grapevine plants showed bacterial cells with the typical morphology of {"}Ca. Cardinium hertigii.{"} This endosymbiont, tentatively designated ST1-C, was found in the cytoplasm of previtellogenic and vitellogenic ovarian cells, in the follicle cells, and in the fat body and salivary glands. In addition, cell morphologies resembling those of {"}Ca. Phytoplasma vitis{"} were detected in the midgut, and specific PCR assays indicated the presence of the phytoplasma in the gut, fat body and salivary glands. These results indicate that ST1-C and {"}Ca. Phytoplasma vitis{"} have a complex life cycle in the body of S. titanus and are colocalized in different organs and tissues.",
author = "Massimo Marzorati and Alberto Alma and Luciano Sacchi and Massimo Pajoro and Simona Palermo and Lorenzo Brusetti and Noura Raddadi and Annalisa Balloi and Rosemarie Tedeschi and Emanuela Clementi and Silvia Corona and Fabio Quaglino and Bianco, {Piero Attilio} and Tiziana Beninati and Claudio Bandi and Daniele Daffonchio",
year = "2006",
month = "2",
doi = "10.1128/AEM.72.2.1467-1475.2006",
language = "English",
volume = "72",
pages = "1467--1475",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera

AU - Marzorati, Massimo

AU - Alma, Alberto

AU - Sacchi, Luciano

AU - Pajoro, Massimo

AU - Palermo, Simona

AU - Brusetti, Lorenzo

AU - Raddadi, Noura

AU - Balloi, Annalisa

AU - Tedeschi, Rosemarie

AU - Clementi, Emanuela

AU - Corona, Silvia

AU - Quaglino, Fabio

AU - Bianco, Piero Attilio

AU - Beninati, Tiziana

AU - Bandi, Claudio

AU - Daffonchio, Daniele

PY - 2006/2

Y1 - 2006/2

N2 - Flavescence dorée (FD) is a grapevine disease that afflicts several wine production areas in Europe, from Portugal to Serbia. FD is caused by a bacterium, "Candidatus Phytoplasma vitis," which is spread throughout the vineyards by a leafhopper, Scaphoideus titanus (Cicadellidae). After collection of S. titanus specimens from FD-contaminated vineyards in three different areas in the Piedmont region of Italy, we performed a survey to characterize the bacterial microflora associated with this insect. Using length heterogeneity PCR with universal primers for bacteria we identified a major peak associated with almost all of the individuals examined (both males and females). Characterization by denaturing gradient gel electrophoresis confirmed the presence of a major band that, after sequencing, showed a 97 to 99% identity with Bacteroidetes symbionts of the "Candidatus Cardinium hertigii" group. In addition, electron microscopy of tissues of S. titanus fed for 3 months on phytoplasma-infected grapevine plants showed bacterial cells with the typical morphology of "Ca. Cardinium hertigii." This endosymbiont, tentatively designated ST1-C, was found in the cytoplasm of previtellogenic and vitellogenic ovarian cells, in the follicle cells, and in the fat body and salivary glands. In addition, cell morphologies resembling those of "Ca. Phytoplasma vitis" were detected in the midgut, and specific PCR assays indicated the presence of the phytoplasma in the gut, fat body and salivary glands. These results indicate that ST1-C and "Ca. Phytoplasma vitis" have a complex life cycle in the body of S. titanus and are colocalized in different organs and tissues.

AB - Flavescence dorée (FD) is a grapevine disease that afflicts several wine production areas in Europe, from Portugal to Serbia. FD is caused by a bacterium, "Candidatus Phytoplasma vitis," which is spread throughout the vineyards by a leafhopper, Scaphoideus titanus (Cicadellidae). After collection of S. titanus specimens from FD-contaminated vineyards in three different areas in the Piedmont region of Italy, we performed a survey to characterize the bacterial microflora associated with this insect. Using length heterogeneity PCR with universal primers for bacteria we identified a major peak associated with almost all of the individuals examined (both males and females). Characterization by denaturing gradient gel electrophoresis confirmed the presence of a major band that, after sequencing, showed a 97 to 99% identity with Bacteroidetes symbionts of the "Candidatus Cardinium hertigii" group. In addition, electron microscopy of tissues of S. titanus fed for 3 months on phytoplasma-infected grapevine plants showed bacterial cells with the typical morphology of "Ca. Cardinium hertigii." This endosymbiont, tentatively designated ST1-C, was found in the cytoplasm of previtellogenic and vitellogenic ovarian cells, in the follicle cells, and in the fat body and salivary glands. In addition, cell morphologies resembling those of "Ca. Phytoplasma vitis" were detected in the midgut, and specific PCR assays indicated the presence of the phytoplasma in the gut, fat body and salivary glands. These results indicate that ST1-C and "Ca. Phytoplasma vitis" have a complex life cycle in the body of S. titanus and are colocalized in different organs and tissues.

UR - http://www.scopus.com/inward/record.url?scp=33144484207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33144484207&partnerID=8YFLogxK

U2 - 10.1128/AEM.72.2.1467-1475.2006

DO - 10.1128/AEM.72.2.1467-1475.2006

M3 - Article

VL - 72

SP - 1467

EP - 1475

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -