A novel class of potential prion drugs: Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles

Hoang Ngoc Ai Tran, Fernanda Sousa, Fabio Moda, Subhra Mandal, Munish Chanana, Chiara Vimercati, Michela Morbin, Silke Krol, Fabrizio Tagliavini, Giuseppe Legname

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Gold nanoparticles coated with oppositely charged polyelectrolytes, such as polyallylamine hydrochloride and polystyrenesulfonate, were examined for potential inhibition of prion protein aggregation and prion (PrPSc) conversion and replication. Different coatings, finishing with a positive or negative layer, were tested, and different numbers of layers were investigated for their ability to interact and reduce the accumulation of PrPSc in scrapie prion infected ScGT1 and ScN2a cells. The particles efficiently hampered the accumulation of PrPSc in ScN2a cells and showed curing effects on ScGT1 cells with a nanoparticle concentration in the picomolar range. Finally, incubation periods of prion-infected mice treated with nanomolar concentrations of gold nanoparticles were significantly longer compared to untreated controls.

Original languageEnglish
Pages (from-to)2724-2732
Number of pages9
JournalNanoscale
Volume2
Issue number12
DOIs
Publication statusPublished - Nov 2010

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Prions
Gold
Multilayers
Nanoparticles
Pharmaceutical Preparations
Polyelectrolytes
Curing
Agglomeration
Coatings

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Ai Tran, H. N., Sousa, F., Moda, F., Mandal, S., Chanana, M., Vimercati, C., ... Legname, G. (2010). A novel class of potential prion drugs: Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles. Nanoscale, 2(12), 2724-2732. https://doi.org/10.1039/c0nr00551g

A novel class of potential prion drugs : Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles. / Ai Tran, Hoang Ngoc; Sousa, Fernanda; Moda, Fabio; Mandal, Subhra; Chanana, Munish; Vimercati, Chiara; Morbin, Michela; Krol, Silke; Tagliavini, Fabrizio; Legname, Giuseppe.

In: Nanoscale, Vol. 2, No. 12, 11.2010, p. 2724-2732.

Research output: Contribution to journalArticle

Ai Tran, HN, Sousa, F, Moda, F, Mandal, S, Chanana, M, Vimercati, C, Morbin, M, Krol, S, Tagliavini, F & Legname, G 2010, 'A novel class of potential prion drugs: Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles', Nanoscale, vol. 2, no. 12, pp. 2724-2732. https://doi.org/10.1039/c0nr00551g
Ai Tran, Hoang Ngoc ; Sousa, Fernanda ; Moda, Fabio ; Mandal, Subhra ; Chanana, Munish ; Vimercati, Chiara ; Morbin, Michela ; Krol, Silke ; Tagliavini, Fabrizio ; Legname, Giuseppe. / A novel class of potential prion drugs : Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles. In: Nanoscale. 2010 ; Vol. 2, No. 12. pp. 2724-2732.
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