A novel class of potential prion drugs: Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles

Hoang Ngoc Ai Tran, Fernanda Sousa, Fabio Moda, Subhra Mandal, Munish Chanana, Chiara Vimercati, Michela Morbin, Silke Krol, Fabrizio Tagliavini, Giuseppe Legname

Research output: Contribution to journalArticle

Abstract

Gold nanoparticles coated with oppositely charged polyelectrolytes, such as polyallylamine hydrochloride and polystyrenesulfonate, were examined for potential inhibition of prion protein aggregation and prion (PrPSc) conversion and replication. Different coatings, finishing with a positive or negative layer, were tested, and different numbers of layers were investigated for their ability to interact and reduce the accumulation of PrPSc in scrapie prion infected ScGT1 and ScN2a cells. The particles efficiently hampered the accumulation of PrPSc in ScN2a cells and showed curing effects on ScGT1 cells with a nanoparticle concentration in the picomolar range. Finally, incubation periods of prion-infected mice treated with nanomolar concentrations of gold nanoparticles were significantly longer compared to untreated controls.

Original languageEnglish
Pages (from-to)2724-2732
Number of pages9
JournalNanoscale
Volume2
Issue number12
DOIs
Publication statusPublished - Nov 2010

ASJC Scopus subject areas

  • Materials Science(all)

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    Ai Tran, H. N., Sousa, F., Moda, F., Mandal, S., Chanana, M., Vimercati, C., Morbin, M., Krol, S., Tagliavini, F., & Legname, G. (2010). A novel class of potential prion drugs: Preliminary in vitro and in vivo data for multilayer coated gold nanoparticles. Nanoscale, 2(12), 2724-2732. https://doi.org/10.1039/c0nr00551g