A novel collection of snRNA-like promoters with tissue-specific transcription properties

Sonia Garritano, Arianna Gigoni, Delfina Costa, Paolo Malatesta, Tullio Florio, Ranieri Cancedda, Aldo Pagano

Research output: Contribution to journalArticle

Abstract

We recently identified a novel dataset of snRNA-like trascriptional units in the human genome. The investigation of a subset of these elements showed that they play relevant roles in physiology and/or pathology. In this work we expand our collection of small RNAs taking advantage of a newly developed algorithm able to identify genome sequence stretches with RNA polymerase (pol) III type 3 promoter features thus constituting putative pol III binding sites. The bioinformatic analysis of a subset of these elements that map in introns of protein-coding genes in antisense configuration suggest their association with alternative splicing, similarly to other recently characterized small RNAs. Interestingly, the analysis of the transcriptional activity of these novel promoters shows that they are active in a cell-type specific manner, in accordance with the emerging body of evidence of a tissue/cell-specific activity of pol III.

Original languageEnglish
Pages (from-to)11323-11332
Number of pages10
JournalInternational Journal of Molecular Sciences
Volume13
Issue number9
DOIs
Publication statusPublished - 2012

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Keywords

  • Alternative splicing
  • Non-coding RNA
  • RNA polymerase iii
  • Small nuclear RNA

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

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