A novel dendritic cell-based vaccination protocol to stimulate immunosurveillance of aggressive cancers

Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, Jessica Dal Col

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A major challenge in the development of a successful tumor vaccination is to break immune tolerance and to sensitize efficiently the immune system toward relevant tumor antigens, thus enabling T-cell-mediated antitumor responses in vivo. Dendritic cell (DC)-based immunotherapy shows the advantage to induce an adaptive immune response against the tumor, with the potential to generate a long-lasting immunological memory able to prevent further relapses and hopefully metastasis. Recently different preclinical studies highlighted the golden opportunity to exploit the features of immunogenic cell death (ICD) to generate ex vivo a highly immunogenic tumor cell lysate as potent antigen formulation for improved DC-based vaccine against aggressive cancers. This chapter focuses on the methods to obtain tumor lysates from cells undergoing ICD to be used for DC pulsing and to test the functionality of the generated DCs for antitumor vaccine development.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages317-333
Number of pages17
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1884
ISSN (Print)1064-3745

Keywords

  • Active immunotherapy
  • Anticancer vaccine
  • Dendritic cells
  • Immunogenic cell death

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Nigro, A., Montico, B., Casolaro, V., & Dal Col, J. (2019). A novel dendritic cell-based vaccination protocol to stimulate immunosurveillance of aggressive cancers. In Methods in Molecular Biology (pp. 317-333). (Methods in Molecular Biology; Vol. 1884). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8885-3_22