A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein

Tania Massignan, Richard S. Stewart, Emiliano Biasini, Isaac H. Solomon, Valentina Bonetto, Roberto Chiesa, David A. Harris

Research output: Contribution to journalArticle

Abstract

In prion diseases, the infectious isoform of the prion protein (PrP Sc) may subvert a normal, physiological activity of the cellular isoform (PrPC). A deletion mutant of the prion protein (Δ105-125) that produces a neonatal lethal phenotype when expressed in transgenic mice provides a window into the normal function of PrPC and how it can be corrupted to produce neurotoxic effects. We report here the surprising and unexpected observation that cells expressing Δ105-125 PrP and related mutants are hypersensitive to the toxic effects of two classes of antibiotics (aminoglycosides and bleomycin analogues) that are commonly used for selection of stably transfected cell lines. This unusual phenomenon mimics several essential features of Δ105-125 PrP toxicity seen in transgenic mice, including rescue by co-expression of wild type PrP. Cells expressing Δ105-125 PrP are susceptible to drug toxicity within minutes, suggesting that the mutant protein enhances cellular accumulation of these cationic compounds. Our results establish a screenable cellular phenotype for the activity of neurotoxic forms of PrP, and they suggest possible mechanisms by which these molecules could produce their pathological effects in vivo.

Original languageEnglish
Pages (from-to)7752-7765
Number of pages14
JournalJournal of Biological Chemistry
Volume285
Issue number10
DOIs
Publication statusPublished - Mar 5 2010

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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