A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA

Riccardo Sarzani, Giorgio Arnaldi, Roberto De Pirro, Paolo Moretti, Stefano Schiaffino, Alessandro Rappelli

Research output: Contribution to journalArticle

Abstract

Degenerate oligonucleotide primers complementary to the highly conserved subdomains III and VIII of subclass III tyrosine kinase receptors (TKr-III) were utilized to amplify rat aortic cDNA by polymerase chain reaction. Most of the cloned DNA products were rat platelet-derived growth factor receptor β and macrophage-colony stimulating growth factor receptor cDNAs. Screening of the clones with probes coding for the receptor-specific kinase insert domain allowed the identification of a novel putative TKr-III cDNA, which hybridized with a ∼6.1 kb mRNA with a distinctive tissue distribution. In situ hybridization on rat tissues and Northern analysis of cultured cells indicate that endothelial cells express a novel putative TKr-III mRNA.

Original languageEnglish
Pages (from-to)706-714
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
Publication statusPublished - Jul 31 1992

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA'. Together they form a unique fingerprint.

Cite this