A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA

Riccardo Sarzani, Giorgio Arnaldi, Roberto De Pirro, Paolo Moretti, Stefano Schiaffino, Alessandro Rappelli

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Degenerate oligonucleotide primers complementary to the highly conserved subdomains III and VIII of subclass III tyrosine kinase receptors (TKr-III) were utilized to amplify rat aortic cDNA by polymerase chain reaction. Most of the cloned DNA products were rat platelet-derived growth factor receptor β and macrophage-colony stimulating growth factor receptor cDNAs. Screening of the clones with probes coding for the receptor-specific kinase insert domain allowed the identification of a novel putative TKr-III cDNA, which hybridized with a ∼6.1 kb mRNA with a distinctive tissue distribution. In situ hybridization on rat tissues and Northern analysis of cultured cells indicate that endothelial cells express a novel putative TKr-III mRNA.

Original languageEnglish
Pages (from-to)706-714
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
Publication statusPublished - Jul 31 1992

Fingerprint

Platelet-Derived Growth Factor Receptors
Protein-Tyrosine Kinases
Rats
Complementary DNA
Macrophage Colony-Stimulating Factor Receptors
Tissue
Vascular Endothelial Growth Factor Receptor-2
Messenger RNA
DNA Primers
Growth Factor Receptors
Macrophages
Polymerase chain reaction
Endothelial cells
Receptor Protein-Tyrosine Kinases
Tissue Distribution
In Situ Hybridization
Cultured Cells
Screening
Phosphotransferases
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA. / Sarzani, Riccardo; Arnaldi, Giorgio; De Pirro, Roberto; Moretti, Paolo; Schiaffino, Stefano; Rappelli, Alessandro.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 2, 31.07.1992, p. 706-714.

Research output: Contribution to journalArticle

Sarzani, Riccardo ; Arnaldi, Giorgio ; De Pirro, Roberto ; Moretti, Paolo ; Schiaffino, Stefano ; Rappelli, Alessandro. / A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 2. pp. 706-714.
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