A novel focal seizure pattern generated in superficial layers of the olfactory cortex

Laura Uva, Stefania Saccucci, Maia Chikhladze, Laura Tassi, Vadym Gnatkovsky, Gloria Milesi, Michela Morbin, Marco de Curtis

Research output: Contribution to journalArticlepeer-review

Abstract

© 2017 the authors. Seizure patterns identified in focal epilepsies caused by diverse etiologies are likely due to different pathogenic mechanisms. We describe here a novel, region-specific focal seizure pattern that mimics seizure activity observed in a subpopulation of patients submitted to presurgical monitoring with intracerebral electrodes. Distinctive seizure-like events (SLEs) are induced in the olfactory regions by acute treatment of both tangential brain slices and the isolated guinea pig brain with the potassium channel blocker 4-aminopyridine. Analysis of field potentials, intracellular activities, and extracellular potassium changes demonstrates that SLEs in the piriform cortex initiate in the superficial layer 1 lacking principal neurons with an activity-dependent increase of extracellular potassium. SLE progression (but not onset) does not require the participation of synaptic transmission and is mediated by diffusion of potassium to deep cortical layers. The novel seizure pattern here described is not observed in other cortical regions; it is proposed to rely on the peculiar organization of the superficial piriform cortex layers, which are characterized by unmyelinated axons and perisynaptic astroglial envelopes. This study reveals a sequence of ictogenic events in the olfactory cortex that were never described before in other cortical structures and supports the notion that altered potassium homeostasis and unmyelinated fibers may represent a potential vehicle for focal ictogenesis.
Original languageEnglish
Pages (from-to)3544-3554
Number of pages11
JournalJournal of Neuroscience
Volume37
Issue number13
DOIs
Publication statusPublished - Mar 29 2017

Keywords

  • 4-aminopyridine
  • Extracellular potassium
  • Human intracerebral EEG recordings
  • In vitro guinea pig brain

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