A novel human homologue of the SH3BGR gene encodes a small protein similar to glutaredoxin 1 of Escherichia coli

M. Mazzocco, P. Arrigo, A. Egeo, M. Maffei, A. Vergano, R. Di Lisi, F. Ghiotto, E. Ciccone, R. Cinti, R. Ravazzolo, P. Scartezzini

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Abstract

Glutaredoxins (GRXs) are ubiquitous GSH-dependent oxidoreductases, which catalyze the reduction of protein-glutathionyl-mixed disulfides and are considered to play an important role in the enzymatic regulation of redox-sensitive proteins. In this paper, we describe the identification and characterization of a new human homologue of the SH3BGR gene, named SH3BGRL3 (SH3 domain binding glutamic acid-rich protein like 3). SH3BGRL3 is widely expressed and codes for a highly conserved small protein, which shows a significant similarity to Glutaredoxin 1 (GRX1) of Escherichia coli and is predicted to belong to the Thioredoxin Superfamily. However, the SH3BGRL3 protein lacks both the conserved served residues, which characterize the enzymatic active site of GRX. This structural feature raises the possibility that SH3BGRL3 could function as an endogenous modulator of GRX biological activity. EGFP-SH3BGRL3 fusion protein expressed in COS-7 cells localizes both to the nucleus and to the cytoplasm. The SH3BGRL3 gene was mapped to chromosome 1p34.3-35.

Original languageEnglish
Pages (from-to)540-545
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume285
Issue number2
DOIs
Publication statusPublished - 2001

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Glutaredoxins
Escherichia coli
Genes
src Homology Domains
Glutamic Acid
Proteins
Thioredoxins
COS Cells
Chromosomes
Bioactivity
Disulfides
Modulators
Oxidation-Reduction
Catalytic Domain
Oxidoreductases
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel human homologue of the SH3BGR gene encodes a small protein similar to glutaredoxin 1 of Escherichia coli. / Mazzocco, M.; Arrigo, P.; Egeo, A.; Maffei, M.; Vergano, A.; Di Lisi, R.; Ghiotto, F.; Ciccone, E.; Cinti, R.; Ravazzolo, R.; Scartezzini, P.

In: Biochemical and Biophysical Research Communications, Vol. 285, No. 2, 2001, p. 540-545.

Research output: Contribution to journalArticle

Mazzocco, M, Arrigo, P, Egeo, A, Maffei, M, Vergano, A, Di Lisi, R, Ghiotto, F, Ciccone, E, Cinti, R, Ravazzolo, R & Scartezzini, P 2001, 'A novel human homologue of the SH3BGR gene encodes a small protein similar to glutaredoxin 1 of Escherichia coli', Biochemical and Biophysical Research Communications, vol. 285, no. 2, pp. 540-545. https://doi.org/10.1006/bbrc.2001.5169
Mazzocco, M. ; Arrigo, P. ; Egeo, A. ; Maffei, M. ; Vergano, A. ; Di Lisi, R. ; Ghiotto, F. ; Ciccone, E. ; Cinti, R. ; Ravazzolo, R. ; Scartezzini, P. / A novel human homologue of the SH3BGR gene encodes a small protein similar to glutaredoxin 1 of Escherichia coli. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 285, No. 2. pp. 540-545.
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