A novel hyaluronan-based gel in laparoscopic adhesion prevention: Preclinical evaluation in an animal model

P. A. De laco, M. Stefanetti, D. Pressato, S. Piana, M. Dona, A. Pavesio, L. Bovicelli

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the effectiveness of a crosslinked hyaluronan solution (ACP gel) in the prevention of postsurgical adhesions in laparoscopic surgery. Design: A randomized blinded study using a rabbit model in laparoscopic surgery. Setting: A standardized surgical trauma in the rabbit uterine horn to induce adhesion formation. Animals: Sixty-four sexually matured female New Zealand white rabbits weighing 2.5 to 3.0 kg and aged 3-4 months. Intervention(s): After trauma, group I (n = 22) received no treatment, group 2 animals (n = 20) received oxidized regenerated cellulose (Interceed [TC7]), in group 3 (n = 22) 5 mL of ACP gel were applied on the lesion. Main Outcome Measure(s): Six weeks after laparoscopy, a laparotomy was performed and the adhesions were scored according to Blauer's scoring system. Result(s): 66% of the unseated animals and 85% of the animals treated with interceed presented with severe adhesions, whereas only 35% of the ACP gel treatment group had significant adhesions. The mean (± SEM) increased adhesion score was 2.24 ± 0.26 in the untreated group, 2.45 ± 0.22 in the interceed group, and was 1.25 ± 0.28 in the ACP gel group. Conclusion(s): This study revealed that ACP gel holds promise as a novel resorbable biomaterial for the reduction of postoperative adhesions after laparoscopic surgery.

Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalFertility and Sterility
Volume69
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Hyaluronic Acid
Animal Models
Gels
Laparoscopy
Rabbits
oxidized cellulose
Wounds and Injuries
Biocompatible Materials
Laparotomy
Outcome Assessment (Health Care)
auto-cross-linked polysaccharide

Keywords

  • Adhesion prevention
  • Animal model
  • Cross-linked hyaluronan
  • Laparoscopic surgery

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

A novel hyaluronan-based gel in laparoscopic adhesion prevention : Preclinical evaluation in an animal model. / De laco, P. A.; Stefanetti, M.; Pressato, D.; Piana, S.; Dona, M.; Pavesio, A.; Bovicelli, L.

In: Fertility and Sterility, Vol. 69, No. 2, 1998, p. 318-323.

Research output: Contribution to journalArticle

De laco, P. A. ; Stefanetti, M. ; Pressato, D. ; Piana, S. ; Dona, M. ; Pavesio, A. ; Bovicelli, L. / A novel hyaluronan-based gel in laparoscopic adhesion prevention : Preclinical evaluation in an animal model. In: Fertility and Sterility. 1998 ; Vol. 69, No. 2. pp. 318-323.
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AB - Objective: To evaluate the effectiveness of a crosslinked hyaluronan solution (ACP gel) in the prevention of postsurgical adhesions in laparoscopic surgery. Design: A randomized blinded study using a rabbit model in laparoscopic surgery. Setting: A standardized surgical trauma in the rabbit uterine horn to induce adhesion formation. Animals: Sixty-four sexually matured female New Zealand white rabbits weighing 2.5 to 3.0 kg and aged 3-4 months. Intervention(s): After trauma, group I (n = 22) received no treatment, group 2 animals (n = 20) received oxidized regenerated cellulose (Interceed [TC7]), in group 3 (n = 22) 5 mL of ACP gel were applied on the lesion. Main Outcome Measure(s): Six weeks after laparoscopy, a laparotomy was performed and the adhesions were scored according to Blauer's scoring system. Result(s): 66% of the unseated animals and 85% of the animals treated with interceed presented with severe adhesions, whereas only 35% of the ACP gel treatment group had significant adhesions. The mean (± SEM) increased adhesion score was 2.24 ± 0.26 in the untreated group, 2.45 ± 0.22 in the interceed group, and was 1.25 ± 0.28 in the ACP gel group. Conclusion(s): This study revealed that ACP gel holds promise as a novel resorbable biomaterial for the reduction of postoperative adhesions after laparoscopic surgery.

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