A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2

Giuliana Napolitano, Daniela Tagliaferri, Salvatore Fusco, Carmine Cirillo, Ilaria De Martino, Martina Addeo, Pellegrino Mazzone, Nicola Antonino Russo, Francesco Natale, Maria Cristina Cardoso, Luciana De Luca, Daniela Lamorte, Francesco La Rocca, Mario De Felice, Geppino Falco

Research output: Contribution to journalArticle

Abstract

Embryonic stem cells (ESCs) fluctuate among different levels of pluripotency defined as metastates. Sporadically, metastable cellular populations convert to a highly pluripotent metastate that resembles the preimplantation two-cell embryos stage (defined as 2C stage) in terms of transcriptome, DNA methylation, and chromatin structure. Recently, we found that the retinoic acid (RA) signaling leads to a robust increase of cells specifically expressing 2C genes, such as members of the Prame family. Here, we show that Gm12794c, one of the most highly upregulated Prame members, and previously identified as a key player for the maintenance of pluripotency, has a functional role in conferring ESCs resistance to RA signaling. In particular, RA-dependent expression of Gm12794c induces a ground state-like metastate, as evaluated by activation of 2C-specific genes, global DNA hypomethylation and rearrangement of chromatin similar to that observed in naive totipotent preimplantation epiblast cells and 2C-like cells. Mechanistically, we demonstrated that Gm12794c inhibits Cdkn1A gene expression through the polycomb repressive complex 2 (PRC2) histone methyltransferase activity. Collectively, our data highlight a molecular mechanism employed by ESCs to counteract retinoic acid differentiation stimuli and contribute to shed light on the molecular mechanisms at grounds of ESCs naive pluripotency-state maintenance.

Original languageEnglish
JournalCell Death and Differentiation
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Polycomb Repressive Complex 2
Methyltransferases
Embryonic Stem Cells
Tretinoin
Chromatin
Maintenance
Germ Layers
Gene Rearrangement
DNA Methylation
Transcriptome
Genes
Embryonic Structures
Gene Expression
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2. / Napolitano, Giuliana; Tagliaferri, Daniela; Fusco, Salvatore; Cirillo, Carmine; De Martino, Ilaria; Addeo, Martina; Mazzone, Pellegrino; Russo, Nicola Antonino; Natale, Francesco; Cardoso, Maria Cristina; De Luca, Luciana; Lamorte, Daniela; La Rocca, Francesco; De Felice, Mario; Falco, Geppino.

In: Cell Death and Differentiation, 01.01.2019.

Research output: Contribution to journalArticle

Napolitano, G, Tagliaferri, D, Fusco, S, Cirillo, C, De Martino, I, Addeo, M, Mazzone, P, Russo, NA, Natale, F, Cardoso, MC, De Luca, L, Lamorte, D, La Rocca, F, De Felice, M & Falco, G 2019, 'A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2', Cell Death and Differentiation. https://doi.org/10.1038/s41418-019-0359-9
Napolitano, Giuliana ; Tagliaferri, Daniela ; Fusco, Salvatore ; Cirillo, Carmine ; De Martino, Ilaria ; Addeo, Martina ; Mazzone, Pellegrino ; Russo, Nicola Antonino ; Natale, Francesco ; Cardoso, Maria Cristina ; De Luca, Luciana ; Lamorte, Daniela ; La Rocca, Francesco ; De Felice, Mario ; Falco, Geppino. / A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2. In: Cell Death and Differentiation. 2019.
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