A novel method for self-administering addicting drugs intracerebroventricularly in a free-choice procedure

Daniela Braida, Walter Virag, Francesco Ottonello, Salvatore Inghilterra, Enzo Gori, Mariaelvina Sala

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper describes a safe method for long term intracerebroventricular (i.c.v.) drug administration. Employing a non-preferred lever to control the self-administration the technique offers advantages over existing experimental methods. To check for any innate preference for one of two levers, male Wistar rats were allowed during the training procedure, to press two levers (L1 and L2) for one hour/day to obtain water as reinforcer for one week in a continuous reinforcement schedule (CRF). One week after surgery, during which a double-guide stainless steel cannula was inserted into both lateral ventricles, rats received 2 μl of sterile cerebrospinal fluid (CEPH) each time they pressed one of two levers during the daily one-hour session. When a stable baseline was reached, rats were divided into three groups on the basis of their lever preference, and submitted to the testing procedure. A potent μ-opiate receptor agonist, etonitazene (0.1-0.2-1 μg/infusion), was always associated with the non-preferred lever for each rat. When no obvious preference was shown for either lever the opiate was firstly delivered by L1 (for 11 days) and then by L2 (for 20 days). The results indicate that, regardless of which lever had been preferred initially, the rats increased the pressing of only the lever associated with the opiate. The daily amount taken increased linearly and was behaviorally active. This model highlights for the first time the reinforcing properties of drugs given i.c.v. in a free-choice situation.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalBrain Research Protocols
Volume3
Issue number2
DOIs
Publication statusPublished - Nov 1998

Fingerprint

Opiate Alkaloids
Pharmaceutical Preparations
Reinforcement Schedule
Self Administration
Lateral Ventricles
Stainless Steel
Opioid Receptors
Cerebrospinal Fluid
Wistar Rats
Water

Keywords

  • Free-choice
  • Intracerebroventricular self-administration
  • Opiates
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A novel method for self-administering addicting drugs intracerebroventricularly in a free-choice procedure. / Braida, Daniela; Virag, Walter; Ottonello, Francesco; Inghilterra, Salvatore; Gori, Enzo; Sala, Mariaelvina.

In: Brain Research Protocols, Vol. 3, No. 2, 11.1998, p. 135-141.

Research output: Contribution to journalArticle

Braida, Daniela ; Virag, Walter ; Ottonello, Francesco ; Inghilterra, Salvatore ; Gori, Enzo ; Sala, Mariaelvina. / A novel method for self-administering addicting drugs intracerebroventricularly in a free-choice procedure. In: Brain Research Protocols. 1998 ; Vol. 3, No. 2. pp. 135-141.
@article{708e0d2765634cf7b3ef9e1a3250740f,
title = "A novel method for self-administering addicting drugs intracerebroventricularly in a free-choice procedure",
abstract = "This paper describes a safe method for long term intracerebroventricular (i.c.v.) drug administration. Employing a non-preferred lever to control the self-administration the technique offers advantages over existing experimental methods. To check for any innate preference for one of two levers, male Wistar rats were allowed during the training procedure, to press two levers (L1 and L2) for one hour/day to obtain water as reinforcer for one week in a continuous reinforcement schedule (CRF). One week after surgery, during which a double-guide stainless steel cannula was inserted into both lateral ventricles, rats received 2 μl of sterile cerebrospinal fluid (CEPH) each time they pressed one of two levers during the daily one-hour session. When a stable baseline was reached, rats were divided into three groups on the basis of their lever preference, and submitted to the testing procedure. A potent μ-opiate receptor agonist, etonitazene (0.1-0.2-1 μg/infusion), was always associated with the non-preferred lever for each rat. When no obvious preference was shown for either lever the opiate was firstly delivered by L1 (for 11 days) and then by L2 (for 20 days). The results indicate that, regardless of which lever had been preferred initially, the rats increased the pressing of only the lever associated with the opiate. The daily amount taken increased linearly and was behaviorally active. This model highlights for the first time the reinforcing properties of drugs given i.c.v. in a free-choice situation.",
keywords = "Free-choice, Intracerebroventricular self-administration, Opiates, Rat",
author = "Daniela Braida and Walter Virag and Francesco Ottonello and Salvatore Inghilterra and Enzo Gori and Mariaelvina Sala",
year = "1998",
month = "11",
doi = "10.1016/S1385-299X(98)00034-8",
language = "English",
volume = "3",
pages = "135--141",
journal = "Brain Research Protocols",
issn = "1385-299X",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - A novel method for self-administering addicting drugs intracerebroventricularly in a free-choice procedure

AU - Braida, Daniela

AU - Virag, Walter

AU - Ottonello, Francesco

AU - Inghilterra, Salvatore

AU - Gori, Enzo

AU - Sala, Mariaelvina

PY - 1998/11

Y1 - 1998/11

N2 - This paper describes a safe method for long term intracerebroventricular (i.c.v.) drug administration. Employing a non-preferred lever to control the self-administration the technique offers advantages over existing experimental methods. To check for any innate preference for one of two levers, male Wistar rats were allowed during the training procedure, to press two levers (L1 and L2) for one hour/day to obtain water as reinforcer for one week in a continuous reinforcement schedule (CRF). One week after surgery, during which a double-guide stainless steel cannula was inserted into both lateral ventricles, rats received 2 μl of sterile cerebrospinal fluid (CEPH) each time they pressed one of two levers during the daily one-hour session. When a stable baseline was reached, rats were divided into three groups on the basis of their lever preference, and submitted to the testing procedure. A potent μ-opiate receptor agonist, etonitazene (0.1-0.2-1 μg/infusion), was always associated with the non-preferred lever for each rat. When no obvious preference was shown for either lever the opiate was firstly delivered by L1 (for 11 days) and then by L2 (for 20 days). The results indicate that, regardless of which lever had been preferred initially, the rats increased the pressing of only the lever associated with the opiate. The daily amount taken increased linearly and was behaviorally active. This model highlights for the first time the reinforcing properties of drugs given i.c.v. in a free-choice situation.

AB - This paper describes a safe method for long term intracerebroventricular (i.c.v.) drug administration. Employing a non-preferred lever to control the self-administration the technique offers advantages over existing experimental methods. To check for any innate preference for one of two levers, male Wistar rats were allowed during the training procedure, to press two levers (L1 and L2) for one hour/day to obtain water as reinforcer for one week in a continuous reinforcement schedule (CRF). One week after surgery, during which a double-guide stainless steel cannula was inserted into both lateral ventricles, rats received 2 μl of sterile cerebrospinal fluid (CEPH) each time they pressed one of two levers during the daily one-hour session. When a stable baseline was reached, rats were divided into three groups on the basis of their lever preference, and submitted to the testing procedure. A potent μ-opiate receptor agonist, etonitazene (0.1-0.2-1 μg/infusion), was always associated with the non-preferred lever for each rat. When no obvious preference was shown for either lever the opiate was firstly delivered by L1 (for 11 days) and then by L2 (for 20 days). The results indicate that, regardless of which lever had been preferred initially, the rats increased the pressing of only the lever associated with the opiate. The daily amount taken increased linearly and was behaviorally active. This model highlights for the first time the reinforcing properties of drugs given i.c.v. in a free-choice situation.

KW - Free-choice

KW - Intracerebroventricular self-administration

KW - Opiates

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0031790363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031790363&partnerID=8YFLogxK

U2 - 10.1016/S1385-299X(98)00034-8

DO - 10.1016/S1385-299X(98)00034-8

M3 - Article

C2 - 9813286

AN - SCOPUS:0031790363

VL - 3

SP - 135

EP - 141

JO - Brain Research Protocols

JF - Brain Research Protocols

SN - 1385-299X

IS - 2

ER -