A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.

C. Traboni; G. Ciliberto; R. Cortese

A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.

C. Traboni, G. Ciliberto, R. Cortese

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article › peer-review

Abstract

We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

Original language	English
Pages (from-to)	415-420
Number of pages	6
Journal	EMBO Journal
Volume	1
Issue number	4
Publication status	Published - 1982

ASJC Scopus subject areas

Cell Biology
Genetics

Fingerprint Dive into the research topics of 'A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.'. Together they form a unique fingerprint.

Cite this

@article{49b6ff38db734df2a053b47f2bcc659a,

title = "A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.",

abstract = "We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.",

author = "C. Traboni and G. Ciliberto and R. Cortese",

year = "1982",

language = "English",

volume = "1",

pages = "415--420",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Nature Publishing Group",

number = "4",

}

TY - JOUR

T1 - A novel method for site-directed mutagenesis

T2 - its application to an eukaryotic tRNAPro gene promoter.

AU - Traboni, C.

AU - Ciliberto, G.

AU - Cortese, R.

PY - 1982

Y1 - 1982

N2 - We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

AB - We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

UR - http://www.scopus.com/inward/record.url?scp=0020338461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020338461&partnerID=8YFLogxK

M3 - Article

C2 - 6329678

AN - SCOPUS:0020338461

VL - 1

SP - 415

EP - 420

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 4

ER -