A novel monoclonal antibody recognizing human thymocytes and B-cell chronic lymphocytic leukemia cells

P. Tassone, P. Bonelli, F. Tuccillo, H. M. Bond, M. C. Turco, G. Morrone, L. Cecco, M. Cerra, S. Venuta

Research output: Contribution to journalArticlepeer-review

Abstract

This paper describes a new murine monoclonal antibody, UN5, raised against human thymocytes. This antibody recognizes a molecule of approximately 45 kDa on thymocytes. Flow cytometric analysis reveals a high intensity of labeling with the majority of thymocytes, whereas only CD20+ cells from peripheral whole-blood samples are weakly stained. Peripheral T cells, granulocytes, platelets and red blood cells do not express this antigen, while monocytes are only weakly labeled by UN5. Furthermore, the UN5 antibody discriminates between different types of B-cell malignancies, reacting with a subgroup of B-cell chronic lymphocytic leukemias and hairy cell leukemias, but not with the other kinds of hematopoietic malignancies tested. Antibody UN5 should prove a useful tool for the study of T-cell precursors and for analysis of both normal and neoplastic B cells.

Original languageEnglish
Pages (from-to)137-146
Number of pages10
JournalImmunology Letters
Volume39
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • B-cell chronic lymphocytic leukemia
  • Monoclonal antibody
  • Monocyte
  • Thymocyte

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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