TY - JOUR
T1 - A novel monoclonal antibody recognizing human thymocytes and B-cell chronic lymphocytic leukemia cells
AU - Tassone, P.
AU - Bonelli, P.
AU - Tuccillo, F.
AU - Bond, H. M.
AU - Turco, M. C.
AU - Morrone, G.
AU - Cecco, L.
AU - Cerra, M.
AU - Venuta, S.
PY - 1994
Y1 - 1994
N2 - This paper describes a new murine monoclonal antibody, UN5, raised against human thymocytes. This antibody recognizes a molecule of approximately 45 kDa on thymocytes. Flow cytometric analysis reveals a high intensity of labeling with the majority of thymocytes, whereas only CD20+ cells from peripheral whole-blood samples are weakly stained. Peripheral T cells, granulocytes, platelets and red blood cells do not express this antigen, while monocytes are only weakly labeled by UN5. Furthermore, the UN5 antibody discriminates between different types of B-cell malignancies, reacting with a subgroup of B-cell chronic lymphocytic leukemias and hairy cell leukemias, but not with the other kinds of hematopoietic malignancies tested. Antibody UN5 should prove a useful tool for the study of T-cell precursors and for analysis of both normal and neoplastic B cells.
AB - This paper describes a new murine monoclonal antibody, UN5, raised against human thymocytes. This antibody recognizes a molecule of approximately 45 kDa on thymocytes. Flow cytometric analysis reveals a high intensity of labeling with the majority of thymocytes, whereas only CD20+ cells from peripheral whole-blood samples are weakly stained. Peripheral T cells, granulocytes, platelets and red blood cells do not express this antigen, while monocytes are only weakly labeled by UN5. Furthermore, the UN5 antibody discriminates between different types of B-cell malignancies, reacting with a subgroup of B-cell chronic lymphocytic leukemias and hairy cell leukemias, but not with the other kinds of hematopoietic malignancies tested. Antibody UN5 should prove a useful tool for the study of T-cell precursors and for analysis of both normal and neoplastic B cells.
KW - B-cell chronic lymphocytic leukemia
KW - Monoclonal antibody
KW - Monocyte
KW - Thymocyte
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U2 - 10.1016/0165-2478(94)90098-1
DO - 10.1016/0165-2478(94)90098-1
M3 - Article
C2 - 8013960
AN - SCOPUS:0028325989
VL - 39
SP - 137
EP - 146
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 2
ER -