A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

Anna De Antoni, Dieter Gallwitz

Research output: Contribution to journalArticle

Abstract

Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kanr marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalGene
Volume246
Issue number1-2
DOIs
Publication statusPublished - Apr 4 2000

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Keywords

  • Cre-recombinase
  • G418 resistance
  • HA
  • loxP
  • MYC
  • PCR-tagging
  • Polyhistidine
  • VSV

ASJC Scopus subject areas

  • Genetics

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