A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

Anna De Antoni, Dieter Gallwitz

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kanr marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalGene
Volume246
Issue number1-2
DOIs
Publication statusPublished - Apr 4 2000

Fingerprint

Saccharomyces cerevisiae
Epitopes
Genes
Polymerase Chain Reaction
Homologous Recombination
Yeasts

Keywords

  • Cre-recombinase
  • G418 resistance
  • HA
  • loxP
  • MYC
  • PCR-tagging
  • Polyhistidine
  • VSV

ASJC Scopus subject areas

  • Genetics

Cite this

A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae. / De Antoni, Anna; Gallwitz, Dieter.

In: Gene, Vol. 246, No. 1-2, 04.04.2000, p. 179-185.

Research output: Contribution to journalArticle

De Antoni, Anna ; Gallwitz, Dieter. / A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae. In: Gene. 2000 ; Vol. 246, No. 1-2. pp. 179-185.
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