A novel near-infrared indocyanine dye-polyethylenimine conjugate allows DNA delivery imaging in vivo

Andrea Masotti, Paola Vicennati, Federico Boschi, Laura Calderan, Andrea Sbarbati, Giancarlo Ortaggi

Research output: Contribution to journalArticlepeer-review

Abstract

Near-infrared (NIR) fluorescence light has been applied to monitor several biological events in vivo since it penetrates tissues more efficiently than visible light. Dyes exhibiting NIR fluorescence and having large Stokes shift are key elements for this promising optical imaging technology. Here, we report the synthesis of a novel conjugate between a near-infrared indocyanine dye and an organic polyamine polymer (polyethylenimine, PEI) (IR820-PEI) with high chemical stability and good optical properties. IR820-PEI absorbs at 665 nm, emits at 780 nm, and displays a large Stokes shift (115 nm). Moreover, the reported conjugate is able to bind DNA, and the delivery process can be monitored in vivo with noninvasive optical imaging techniques. These characteristics make IR820-PEI one of the most effective and versatile indocyanine dye polymeric-conjugate reported so far.

Original languageEnglish
Pages (from-to)983-987
Number of pages5
JournalBioconjugate Chemistry
Volume19
Issue number5
DOIs
Publication statusPublished - May 2008

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Clinical Biochemistry
  • Chemistry(all)
  • Organic Chemistry

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