A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors

C. Voena, M. Ladetto, M. Astolfi, D. Provan, J. G. Gribben, M. Boccadoro, A. Pileri, P. Corradini

Research output: Contribution to journalArticle

Abstract

Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83%). Sensitivity using DNA or cDNA templates was 10-5 and -6, respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors.

Original languageEnglish
Pages (from-to)1793-1798
Number of pages6
JournalLeukemia
Volume11
Issue number10
Publication statusPublished - 1997

Keywords

  • B cell malignancies
  • IgH nested-PCR
  • Minimal residual disease

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

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    Voena, C., Ladetto, M., Astolfi, M., Provan, D., Gribben, J. G., Boccadoro, M., Pileri, A., & Corradini, P. (1997). A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. Leukemia, 11(10), 1793-1798.