A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors

C. Voena, M. Ladetto, M. Astolfi, D. Provan, J. G. Gribben, M. Boccadoro, A. Pileri, P. Corradini

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83%). Sensitivity using DNA or cDNA templates was 10-5 and -6, respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors.

Original languageEnglish
Pages (from-to)1793-1798
Number of pages6
JournalLeukemia
Volume11
Issue number10
Publication statusPublished - 1997

Fingerprint

Immunoglobulin Heavy Chain Genes
Residual Neoplasm
B-Lymphocytes
Immunoglobulin Heavy Chains
Polymerase Chain Reaction
Neoplasms
Complementarity Determining Regions
T-Cell Receptor Genes
Genetic Translocation
B-Cell Chronic Lymphocytic Leukemia
Multiple Myeloma
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Non-Hodgkin's Lymphoma
Complementary DNA
DNA
Genes

Keywords

  • B cell malignancies
  • IgH nested-PCR
  • Minimal residual disease

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Voena, C., Ladetto, M., Astolfi, M., Provan, D., Gribben, J. G., Boccadoro, M., ... Corradini, P. (1997). A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. Leukemia, 11(10), 1793-1798.

A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. / Voena, C.; Ladetto, M.; Astolfi, M.; Provan, D.; Gribben, J. G.; Boccadoro, M.; Pileri, A.; Corradini, P.

In: Leukemia, Vol. 11, No. 10, 1997, p. 1793-1798.

Research output: Contribution to journalArticle

Voena, C, Ladetto, M, Astolfi, M, Provan, D, Gribben, JG, Boccadoro, M, Pileri, A & Corradini, P 1997, 'A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors', Leukemia, vol. 11, no. 10, pp. 1793-1798.
Voena C, Ladetto M, Astolfi M, Provan D, Gribben JG, Boccadoro M et al. A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. Leukemia. 1997;11(10):1793-1798.
Voena, C. ; Ladetto, M. ; Astolfi, M. ; Provan, D. ; Gribben, J. G. ; Boccadoro, M. ; Pileri, A. ; Corradini, P. / A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. In: Leukemia. 1997 ; Vol. 11, No. 10. pp. 1793-1798.
@article{7536c32e31474bc29f14d8b6a7165a8a,
title = "A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors",
abstract = "Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83{\%}). Sensitivity using DNA or cDNA templates was 10-5 and -6, respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors.",
keywords = "B cell malignancies, IgH nested-PCR, Minimal residual disease",
author = "C. Voena and M. Ladetto and M. Astolfi and D. Provan and Gribben, {J. G.} and M. Boccadoro and A. Pileri and P. Corradini",
year = "1997",
language = "English",
volume = "11",
pages = "1793--1798",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors

AU - Voena, C.

AU - Ladetto, M.

AU - Astolfi, M.

AU - Provan, D.

AU - Gribben, J. G.

AU - Boccadoro, M.

AU - Pileri, A.

AU - Corradini, P.

PY - 1997

Y1 - 1997

N2 - Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83%). Sensitivity using DNA or cDNA templates was 10-5 and -6, respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors.

AB - Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83%). Sensitivity using DNA or cDNA templates was 10-5 and -6, respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors.

KW - B cell malignancies

KW - IgH nested-PCR

KW - Minimal residual disease

UR - http://www.scopus.com/inward/record.url?scp=0030698409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030698409&partnerID=8YFLogxK

M3 - Article

C2 - 9324303

AN - SCOPUS:0030698409

VL - 11

SP - 1793

EP - 1798

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 10

ER -