A novel pattern of fast calcium oscillations points to calcium and electrical activity cross-talk in rat chromaffin cells

M. Micheletti, A. Brioschi, R. Fesce, F. Grohovaz

Research output: Contribution to journalArticlepeer-review

Abstract

Slow oscillations of cytosolic calcium ion concentration - [Ca 2+]c - typically originate from release by intracellular stores, but in some cell types can be triggered and sustained by Ca2+ influx as well. In this study we simultaneously monitored changes in [Ca 2+]c and in the electrical activity of the cell membrane by combining indo-1 and patch-clamp measurements in single rat chromaffin cells. By this approach we observed a novel type of spontaneous [Ca2+] c oscillations, much faster than those previously described in these cells. These oscillations are triggered and sustained by complex electrical activity (slow action potentials and spike bursts), require Ca2+ influx and do not involve release from intracellular stores. The possible physiological implications of this new pathway of intracellular signalling are discussed.

Original languageEnglish
Pages (from-to)95-104
Number of pages10
JournalCellular and Molecular Life Sciences
Volume62
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • Action-potential clamp
  • Calcium oscillations
  • Electrophysiology
  • Indo-1 microspectrofluorometry
  • Ionic channels

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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