TY - JOUR
T1 - A novel polyclonal antibody library for expression profiling of poorly characterized, membrane and secreted human proteins
AU - Grifantini, Renata
AU - Pagani, Massimiliano
AU - Pierleoni, Andrea
AU - Grandi, Alberto
AU - Parri, Matteo
AU - Campagnoli, Susanna
AU - Pileri, Piero
AU - Cattaneo, Davide
AU - Canidio, Elena
AU - Pontillo, Angela
AU - De Camilli, Elisa
AU - Bresciani, Alberto
AU - Marinoni, Federica
AU - Pedrazzoli, Eros
AU - Nogarotto, Renzo
AU - Abrignani, Sergio
AU - Viale, Giuseppe
AU - Sarmientos, Paolo
AU - Grandi, Guido
PY - 2011/12/21
Y1 - 2011/12/21
N2 - The YOMICS™ antibody library (http://www.yomics.com/) presented in this article is a new collection of 1559 murine polyclonal antibodies specific for 1287 distinct human proteins. This antibody library is designed to target marginally characterized membrane-associated and secreted proteins. It was generated against human proteins annotated as transmembrane or secreted in GenBank, EnsEMBL, Vega and Uniprot databases, described in no or very few dedicated PubMed-linked publications. The selected proteins/protein regions were expressed in E. coli, purified and used to raise antibodies in the mouse. The capability of YOMICS™ antibodies to specifically recognize their target proteins either as recombinant form or as expressed in cells and tissues was confirmed through several experimental approaches, including Western blot, confocal microscopy and immunohistochemistry (IHC). Moreover, to show the applicability of the library for biomarker investigation by IHC, five antibodies against proteins either known to be expressed in some cancers or homologous to tumor-associated proteins were tested on tissue microarrays carrying tumor and normal tissues from breast, colon, lung, ovary and prostate. A consistent differential expression in cancer was observed. Our results indicate that the YOMICS™ antibody library is a tool for systematic protein expression profile analysis that nicely complements the already available commercial antibody collections.
AB - The YOMICS™ antibody library (http://www.yomics.com/) presented in this article is a new collection of 1559 murine polyclonal antibodies specific for 1287 distinct human proteins. This antibody library is designed to target marginally characterized membrane-associated and secreted proteins. It was generated against human proteins annotated as transmembrane or secreted in GenBank, EnsEMBL, Vega and Uniprot databases, described in no or very few dedicated PubMed-linked publications. The selected proteins/protein regions were expressed in E. coli, purified and used to raise antibodies in the mouse. The capability of YOMICS™ antibodies to specifically recognize their target proteins either as recombinant form or as expressed in cells and tissues was confirmed through several experimental approaches, including Western blot, confocal microscopy and immunohistochemistry (IHC). Moreover, to show the applicability of the library for biomarker investigation by IHC, five antibodies against proteins either known to be expressed in some cancers or homologous to tumor-associated proteins were tested on tissue microarrays carrying tumor and normal tissues from breast, colon, lung, ovary and prostate. A consistent differential expression in cancer was observed. Our results indicate that the YOMICS™ antibody library is a tool for systematic protein expression profile analysis that nicely complements the already available commercial antibody collections.
KW - Antibody collection
KW - Human proteins
KW - Immunohistochemistry
KW - Tissue microarray
UR - http://www.scopus.com/inward/record.url?scp=82355169497&partnerID=8YFLogxK
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U2 - 10.1016/j.jprot.2011.08.018
DO - 10.1016/j.jprot.2011.08.018
M3 - Article
C2 - 21920474
AN - SCOPUS:82355169497
VL - 75
SP - 532
EP - 547
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 2
ER -