Abstract
Easily reproducible animal models that allow for study of the biology of chronic lymphocytic leukemia (CLL) and to test new therapeutic agents have been very difficult to establish. We have developed a novel transplantable xenograft murine model of CLL by engrafting the CLL cell line MEC1 into Rag2 -/-γc-/- mice. These mice lack B, T, and natural killer (NK) cells, and, in contrast to nude mice that retain NK cells, appear to be optimal recipient for MEC1 cells, which were successfully transplanted through either subcutaneous or intravenous routes. The result is a novel in vivo model that has systemic involvement, develops very rapidly, allows the measurement of tumor burden, and has 100% engraftment efficiency. This model closely resembles aggressive human CLL and could be very useful for evaluating both the biologic basis of CLL growth and dissemination as well as the efficacy of new therapeutic agents.
| Original language | English |
|---|---|
| Pages (from-to) | 1605-1609 |
| Number of pages | 5 |
| Journal | Blood |
| Volume | 115 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Feb 25 2010 |
Fingerprint
ASJC Scopus subject areas
- Hematology
- Biochemistry
- Cell Biology
- Immunology
Cite this
A novel Rag2-/-γc -/--xenograft model of human CLL. / Bertilaccio, Maria Teresa Sabrina; Scielzo, Cristina; Simonetti, Giorgia; Ponzoni, Maurilio; Apollonio, Benedetta; Fazi, Claudia; Scarfò, Lydia; Rocchi, Martina; Muzio, Marta; Caligaris-Cappio, Federico; Ghia, Paolo.
In: Blood, Vol. 115, No. 8, 25.02.2010, p. 1605-1609.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel Rag2-/-γc -/--xenograft model of human CLL
AU - Bertilaccio, Maria Teresa Sabrina
AU - Scielzo, Cristina
AU - Simonetti, Giorgia
AU - Ponzoni, Maurilio
AU - Apollonio, Benedetta
AU - Fazi, Claudia
AU - Scarfò, Lydia
AU - Rocchi, Martina
AU - Muzio, Marta
AU - Caligaris-Cappio, Federico
AU - Ghia, Paolo
PY - 2010/2/25
Y1 - 2010/2/25
N2 - Easily reproducible animal models that allow for study of the biology of chronic lymphocytic leukemia (CLL) and to test new therapeutic agents have been very difficult to establish. We have developed a novel transplantable xenograft murine model of CLL by engrafting the CLL cell line MEC1 into Rag2 -/-γc-/- mice. These mice lack B, T, and natural killer (NK) cells, and, in contrast to nude mice that retain NK cells, appear to be optimal recipient for MEC1 cells, which were successfully transplanted through either subcutaneous or intravenous routes. The result is a novel in vivo model that has systemic involvement, develops very rapidly, allows the measurement of tumor burden, and has 100% engraftment efficiency. This model closely resembles aggressive human CLL and could be very useful for evaluating both the biologic basis of CLL growth and dissemination as well as the efficacy of new therapeutic agents.
AB - Easily reproducible animal models that allow for study of the biology of chronic lymphocytic leukemia (CLL) and to test new therapeutic agents have been very difficult to establish. We have developed a novel transplantable xenograft murine model of CLL by engrafting the CLL cell line MEC1 into Rag2 -/-γc-/- mice. These mice lack B, T, and natural killer (NK) cells, and, in contrast to nude mice that retain NK cells, appear to be optimal recipient for MEC1 cells, which were successfully transplanted through either subcutaneous or intravenous routes. The result is a novel in vivo model that has systemic involvement, develops very rapidly, allows the measurement of tumor burden, and has 100% engraftment efficiency. This model closely resembles aggressive human CLL and could be very useful for evaluating both the biologic basis of CLL growth and dissemination as well as the efficacy of new therapeutic agents.
UR - http://www.scopus.com/inward/record.url?scp=77949890578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949890578&partnerID=8YFLogxK
U2 - 10.1182/blood-2009-05-223586
DO - 10.1182/blood-2009-05-223586
M3 - Article
C2 - 20018917
AN - SCOPUS:77949890578
VL - 115
SP - 1605
EP - 1609
JO - Blood
JF - Blood
SN - 0006-4971
IS - 8
ER -