A novel Rag2-/-γc -/--xenograft model of human CLL

Maria Teresa Sabrina Bertilaccio, Cristina Scielzo, Giorgia Simonetti, Maurilio Ponzoni, Benedetta Apollonio, Claudia Fazi, Lydia Scarfò, Martina Rocchi, Marta Muzio, Federico Caligaris-Cappio, Paolo Ghia

Research output: Contribution to journalArticlepeer-review

Abstract

Easily reproducible animal models that allow for study of the biology of chronic lymphocytic leukemia (CLL) and to test new therapeutic agents have been very difficult to establish. We have developed a novel transplantable xenograft murine model of CLL by engrafting the CLL cell line MEC1 into Rag2 -/-γc-/- mice. These mice lack B, T, and natural killer (NK) cells, and, in contrast to nude mice that retain NK cells, appear to be optimal recipient for MEC1 cells, which were successfully transplanted through either subcutaneous or intravenous routes. The result is a novel in vivo model that has systemic involvement, develops very rapidly, allows the measurement of tumor burden, and has 100% engraftment efficiency. This model closely resembles aggressive human CLL and could be very useful for evaluating both the biologic basis of CLL growth and dissemination as well as the efficacy of new therapeutic agents.

Original languageEnglish
Pages (from-to)1605-1609
Number of pages5
JournalBlood
Volume115
Issue number8
DOIs
Publication statusPublished - Feb 25 2010

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

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