A novel RGDS-analog inhibits angiogenesis in vitro and in vivo

M. S. Aguzzi, F. Facchiano, D. Ribatti, R. Gaeta, R. Casadio, I. Rossi, M. C. Capogrossi, A. Facchiano

Research output: Contribution to journalArticle

Abstract

In this study the anti-angiogenic action of a novel non-peptide RGDS-analog named RAM was tested in vitro and in vivo. RAM inhibited FGF-2-induced chemotaxis by 80% in an adhesion-independent way. Further, it induced HUVEC-apoptosis in collagen-seeded HUVEC, indicating that such pro-apoptotic effect was adhesion-independent. In vivo studies revealed that RAM inhibited FGF-2 induced angiogenesis by 60% in the mouse Matrigel-assay and in the chicken-egg chorion-allantoic membrane assay. Finally, RAM was markedly more stable in serum as compared to the template RGDS and after 24 h incubation in 100% serum was significantly more active than RGDS. Taken together these results show that RAM exerts anti-chemotactic and pro-apoptotic effects, by an unexpected adhesion-independent mechanism, as we have recently shown for the template RGDS molecule [Blood 103 (2004) 4180], and has in vivo relevant anti-angiogenic properties, with marked stability in serum; therefore, RAM represents a novel promising anti-angiogenic molecule.

Original languageEnglish
Pages (from-to)809-814
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number4
DOIs
Publication statusPublished - Sep 3 2004

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Fibroblast Growth Factor 2
Allantois
Serum
Chorion
Adhesion
Chemotaxis
Ovum
Chickens
Assays
Collagen
Apoptosis
Molecules
In Vitro Techniques
Blood
Membranes

Keywords

  • Adhesion
  • Angiogenesis
  • Apoptosis
  • Cell-matrix interactions
  • Endothelial cells
  • Integrins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel RGDS-analog inhibits angiogenesis in vitro and in vivo. / Aguzzi, M. S.; Facchiano, F.; Ribatti, D.; Gaeta, R.; Casadio, R.; Rossi, I.; Capogrossi, M. C.; Facchiano, A.

In: Biochemical and Biophysical Research Communications, Vol. 321, No. 4, 03.09.2004, p. 809-814.

Research output: Contribution to journalArticle

Aguzzi, MS, Facchiano, F, Ribatti, D, Gaeta, R, Casadio, R, Rossi, I, Capogrossi, MC & Facchiano, A 2004, 'A novel RGDS-analog inhibits angiogenesis in vitro and in vivo', Biochemical and Biophysical Research Communications, vol. 321, no. 4, pp. 809-814. https://doi.org/10.1016/j.bbrc.2004.07.036
Aguzzi, M. S. ; Facchiano, F. ; Ribatti, D. ; Gaeta, R. ; Casadio, R. ; Rossi, I. ; Capogrossi, M. C. ; Facchiano, A. / A novel RGDS-analog inhibits angiogenesis in vitro and in vivo. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 321, No. 4. pp. 809-814.
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