Abstract
To investigate the genetic contribution to familial similarity in longevity, we set up a novel experimental design where cousin-pairs born from siblings who were concordant or discordant for the longevity trait were analyzed. To check this design, two chromosomal regions already known to encompass longevity-related genes were examined: 6p21.3 (genes TNFα, TNFβ, HSP70.1) and 11p15.5 (genes SIRT3, HRAS1, IGF2, INS, TH). Population pools of 1.6, 2.3 and 2.0 million inhabitants were screened, respectively, in Denmark, France and Italy to identify families matching the design requirements. A total of 234 trios composed by one centenarian, his/her child and a child of his/her concordant or discordant sib were collected. By using population-specific allele frequencies, we reconstructed haplotype phase and estimated the likelihood of Identical By Descent (IBD) haplotype sharing in cousin-pairs born from concordant and discordant siblings. In addition, we analyzed haplotype transmission from centenarians to offspring, and a statistically significant Transmission Ratio Distortion (TRD) was observed for both chromosomal regions in the discordant families (P=0.007 for 6p21.3 and P=0.015 for 11p15.5). In concordant families, a marginally significant TRD was observed at 6p21.3 only (P=0.06). Although no significant difference emerged between the two groups of cousin-pairs, our study gave new insights on the hindrances to recruiting a suitable sample to obtain significant IBD data on longevity-related chromosomal regions. This will allow to dimension future sampling campaigns to study-genetic basis of human longevity.
| Original language | English |
|---|---|
| Pages (from-to) | 236-242 |
| Number of pages | 7 |
| Journal | European Journal of Human Genetics |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2008 |
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ASJC Scopus subject areas
- Genetics(clinical)
Cite this
A novel sampling design to explore gene-longevity associations : The ECHA study. / De Rango, Francesco; Dato, Serena; Bellizzi, Dina; Rose, Giuseppina; Marzi, Erika; Cavallone, Luca; Franceschi, Claudio; Skytthe, Axel; Jeune, Bernard; Cournil, Amandine; Robine, Jean Marie; Gampe, Jutta; Vaupel, James W.; Mari, Vincenzo; Feraco, Emidio; Passarino, Giuseppe; Novelletto, Andrea; De Benedictis, Giovanna.
In: European Journal of Human Genetics, Vol. 16, No. 2, 02.2008, p. 236-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel sampling design to explore gene-longevity associations
T2 - The ECHA study
AU - De Rango, Francesco
AU - Dato, Serena
AU - Bellizzi, Dina
AU - Rose, Giuseppina
AU - Marzi, Erika
AU - Cavallone, Luca
AU - Franceschi, Claudio
AU - Skytthe, Axel
AU - Jeune, Bernard
AU - Cournil, Amandine
AU - Robine, Jean Marie
AU - Gampe, Jutta
AU - Vaupel, James W.
AU - Mari, Vincenzo
AU - Feraco, Emidio
AU - Passarino, Giuseppe
AU - Novelletto, Andrea
AU - De Benedictis, Giovanna
PY - 2008/2
Y1 - 2008/2
N2 - To investigate the genetic contribution to familial similarity in longevity, we set up a novel experimental design where cousin-pairs born from siblings who were concordant or discordant for the longevity trait were analyzed. To check this design, two chromosomal regions already known to encompass longevity-related genes were examined: 6p21.3 (genes TNFα, TNFβ, HSP70.1) and 11p15.5 (genes SIRT3, HRAS1, IGF2, INS, TH). Population pools of 1.6, 2.3 and 2.0 million inhabitants were screened, respectively, in Denmark, France and Italy to identify families matching the design requirements. A total of 234 trios composed by one centenarian, his/her child and a child of his/her concordant or discordant sib were collected. By using population-specific allele frequencies, we reconstructed haplotype phase and estimated the likelihood of Identical By Descent (IBD) haplotype sharing in cousin-pairs born from concordant and discordant siblings. In addition, we analyzed haplotype transmission from centenarians to offspring, and a statistically significant Transmission Ratio Distortion (TRD) was observed for both chromosomal regions in the discordant families (P=0.007 for 6p21.3 and P=0.015 for 11p15.5). In concordant families, a marginally significant TRD was observed at 6p21.3 only (P=0.06). Although no significant difference emerged between the two groups of cousin-pairs, our study gave new insights on the hindrances to recruiting a suitable sample to obtain significant IBD data on longevity-related chromosomal regions. This will allow to dimension future sampling campaigns to study-genetic basis of human longevity.
AB - To investigate the genetic contribution to familial similarity in longevity, we set up a novel experimental design where cousin-pairs born from siblings who were concordant or discordant for the longevity trait were analyzed. To check this design, two chromosomal regions already known to encompass longevity-related genes were examined: 6p21.3 (genes TNFα, TNFβ, HSP70.1) and 11p15.5 (genes SIRT3, HRAS1, IGF2, INS, TH). Population pools of 1.6, 2.3 and 2.0 million inhabitants were screened, respectively, in Denmark, France and Italy to identify families matching the design requirements. A total of 234 trios composed by one centenarian, his/her child and a child of his/her concordant or discordant sib were collected. By using population-specific allele frequencies, we reconstructed haplotype phase and estimated the likelihood of Identical By Descent (IBD) haplotype sharing in cousin-pairs born from concordant and discordant siblings. In addition, we analyzed haplotype transmission from centenarians to offspring, and a statistically significant Transmission Ratio Distortion (TRD) was observed for both chromosomal regions in the discordant families (P=0.007 for 6p21.3 and P=0.015 for 11p15.5). In concordant families, a marginally significant TRD was observed at 6p21.3 only (P=0.06). Although no significant difference emerged between the two groups of cousin-pairs, our study gave new insights on the hindrances to recruiting a suitable sample to obtain significant IBD data on longevity-related chromosomal regions. This will allow to dimension future sampling campaigns to study-genetic basis of human longevity.
UR - http://www.scopus.com/inward/record.url?scp=38349176358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38349176358&partnerID=8YFLogxK
U2 - 10.1038/sj.ejhg.5201950
DO - 10.1038/sj.ejhg.5201950
M3 - Article
C2 - 17989723
AN - SCOPUS:38349176358
VL - 16
SP - 236
EP - 242
JO - European Journal of Human Genetics
JF - European Journal of Human Genetics
SN - 1018-4813
IS - 2
ER -