A novel semi-automated in situ hybridisation protocol for microRNA detection in paraffin embedded tissue sections

Ambra Vittoria Gualeni, Chiara Costanza Volpi, Antonino Carbone, Annunziata Gloghini

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that modulate gene expression by binding to complementary sequences on target messenger RNA transcripts. In situ hybridisation (ISH) methods have been applied to the study of miRNA in tissue samples in order to understand which is the source of the miRNA of interest. In this paper, the authors describe a novel semi-automated bright field ISH method to visualise miRNAs in formalin fixed paraffin embedded tissue sections. The relevance of this work resides in the use of 3,30-diaminobenzidine and peroxidase as the detection method, which provides a good defined deposition within tissues This method, which reveals the cells of origin of specific miRNAs, will enable investigators to further explore the biological role of miRNAs.

Original languageEnglish
Pages (from-to)661-664
Number of pages4
JournalJournal of Clinical Pathology
Volume68
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

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