A novel splice variant of the human dicer gene is expressed in neuroblastoma cells

Nicoletta Potenza, Umberto Papa, Paola Scaruffi, Nicola Mosca, Gian Paolo Tonini, Aniello Russo

Research output: Contribution to journalArticlepeer-review

Abstract

Dicer is a ribonuclease playing a key role in the biogenesis of microRNAs and small interfering RNAs. Here we report the identification of a novel splice variant of human dicer gene, the first one bearing a modified coding sequence. It encodes a truncated protein, t-Dicer that lacks the dsRNA-binding domain and is defective in one of the two RNase III catalytic centers. The splice variant was found in neuroblastoma cells and in cells induced to neuronal differentiation, whereas it was not detectable in other cell lines or in normal tissues. Interestingly, it occurred in primary neuroblastic tumors, mainly in stroma poor neuroblastomas.

Original languageEnglish
Pages (from-to)3452-3457
Number of pages6
JournalFEBS Letters
Volume584
Issue number15
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Alternative splicing
  • Dicer
  • MiRNA
  • Neural differentiation
  • Neuroblastoma
  • RNase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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