A paracrine circuit in the regulation of the proliferation of macrophages infiltrating murine sarcomas

Barbara Bottazzi, Eugenio Erba, Nadia Nobili, Francesca Fazioli, Alessandro Rambaldi, Alberto Mantovani

Research output: Contribution to journalArticle

Abstract

Tumor-associated macrophages (TAM) isolated from two murine sarcomas (mFS6 and MN/MCA1) had high levels of proliferative activity (7 to 11% of cells in S phase) compared to peritoneal macrophages (1 to 2% of cells in S phase). In an effort to elucidate the mechanisms responsible for the proliferative activity of TAM, expression of c-fms and macrophage (M)-CSF was investigated in TAM and sarcoma cells. TAM had high levels of mRNA transcripts of the c-fms protooncogene, which encodes a tyrosine kinase probably identical to the M-CSF receptor, but did not express M-CSF transcripts whereas sarcoma cells had high levels of M-CSF mRNA. Sarcoma cell conditioned medium had M-CSF activity on bone marrow cells and induced proliferation of peritoneal exudate and bone marrow-derived macrophages. These activities were blocked by anti-M-CSF antibodies. These findings outline a paracrine circuit in the regulation of TAM proliferation, involving M-CSF, secreted by sarcoma cells and acting on c-fms expressing TAM. Inasmuch as TAM from these murine sarcomas have tumor growth promoting activity, a "ping pong" reciprocal feeding interaction may occur between macrophages and neoplastic cells in these tumors.

Original languageEnglish
Pages (from-to)2409-2412
Number of pages4
JournalJournal of Immunology
Volume144
Issue number6
Publication statusPublished - Mar 15 1990

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Sarcoma
Macrophages
Macrophage Colony-Stimulating Factor
Neoplasms
S Phase
Macrophage Colony-Stimulating Factor Receptors
Messenger RNA
Peritoneal Macrophages
Exudates and Transudates
Conditioned Culture Medium
Bone Marrow Cells
Protein-Tyrosine Kinases
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

A paracrine circuit in the regulation of the proliferation of macrophages infiltrating murine sarcomas. / Bottazzi, Barbara; Erba, Eugenio; Nobili, Nadia; Fazioli, Francesca; Rambaldi, Alessandro; Mantovani, Alberto.

In: Journal of Immunology, Vol. 144, No. 6, 15.03.1990, p. 2409-2412.

Research output: Contribution to journalArticle

Bottazzi, Barbara ; Erba, Eugenio ; Nobili, Nadia ; Fazioli, Francesca ; Rambaldi, Alessandro ; Mantovani, Alberto. / A paracrine circuit in the regulation of the proliferation of macrophages infiltrating murine sarcomas. In: Journal of Immunology. 1990 ; Vol. 144, No. 6. pp. 2409-2412.
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AU - Erba, Eugenio

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AU - Rambaldi, Alessandro

AU - Mantovani, Alberto

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