TY - JOUR
T1 - A Perspective on Extracellular Vesicles Proteomics
AU - Rosa-Fernandes, Livia
AU - Rocha, Victória Bombarda
AU - Carregari, Victor Corasolla
AU - Urbani, Andrea
AU - Palmisano, Giuseppe
PY - 2017
Y1 - 2017
N2 - Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
AB - Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources. Here we will provide a perspective view on the achievements and challenges on EVs proteome characterization using mass spectrometry. A detailed bioinformatics approach will help us to picture the molecular fingerprint of EVs and understand better their pathophysiological function.
KW - Journal Article
KW - Review
U2 - 10.3389/fchem.2017.00102
DO - 10.3389/fchem.2017.00102
M3 - Review article
C2 - 29209607
VL - 5
SP - 102
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
SN - 2296-2646
ER -