A pilot study to investigate the balance between proteases and α1-antitrypsin in bronchoalveolar lavage fluid of lung transplant recipients

Maddalena Cagnone, Davide Piloni, Ilaria Ferrarotti, Monica Di Venere, Simona Viglio, Sara Magni, Anna Bardoni, Roberta Salvini, Marco Fumagalli, Paolo Iadarola, Sabrina Martinello, Federica Meloni

Research output: Contribution to journalArticle

Abstract

The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count. The results of these experiments demonstrated, for the first time, the presence of the complex between HNE and AAT in a number of BALf samples. The lack of this complex in a few specimens analyzed was investigated in relation to a patient’s lung inflammation. The neutrophil count and the determination of HNE and AAT activities allowed us to speculate that the presence of the complex correlated with the level of lung inflammation.

Original languageEnglish
Article number5
JournalHigh-Throughput
Volume8
Issue number1
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

Leukocyte Elastase
Transplants
Bronchoalveolar Lavage Fluid
Peptide Hydrolases
Protease Inhibitors
Lung
Fluids
Elastin
Pneumonia
Neutrophils
Liquid chromatography
Bronchiolitis Obliterans
Electrophoresis
Serine Proteases
Liquid Chromatography
Degradation
Western Blotting
Maintenance
Transplant Recipients
Experiments

Keywords

  • Alpha 1-antitrypsin
  • Bronchiolitis obliterans syndrome
  • Bronchoalveolar lavage fluid
  • Elastase
  • Lung
  • Neutrophils

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomedical Engineering

Cite this

A pilot study to investigate the balance between proteases and α1-antitrypsin in bronchoalveolar lavage fluid of lung transplant recipients. / Cagnone, Maddalena; Piloni, Davide; Ferrarotti, Ilaria; Di Venere, Monica; Viglio, Simona; Magni, Sara; Bardoni, Anna; Salvini, Roberta; Fumagalli, Marco; Iadarola, Paolo; Martinello, Sabrina; Meloni, Federica.

In: High-Throughput, Vol. 8, No. 1, 5, 01.03.2019.

Research output: Contribution to journalArticle

Cagnone, Maddalena ; Piloni, Davide ; Ferrarotti, Ilaria ; Di Venere, Monica ; Viglio, Simona ; Magni, Sara ; Bardoni, Anna ; Salvini, Roberta ; Fumagalli, Marco ; Iadarola, Paolo ; Martinello, Sabrina ; Meloni, Federica. / A pilot study to investigate the balance between proteases and α1-antitrypsin in bronchoalveolar lavage fluid of lung transplant recipients. In: High-Throughput. 2019 ; Vol. 8, No. 1.
@article{1cbeb8e3ad8544e5b2a3132278774660,
title = "A pilot study to investigate the balance between proteases and α1-antitrypsin in bronchoalveolar lavage fluid of lung transplant recipients",
abstract = "The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count. The results of these experiments demonstrated, for the first time, the presence of the complex between HNE and AAT in a number of BALf samples. The lack of this complex in a few specimens analyzed was investigated in relation to a patient’s lung inflammation. The neutrophil count and the determination of HNE and AAT activities allowed us to speculate that the presence of the complex correlated with the level of lung inflammation.",
keywords = "Alpha 1-antitrypsin, Bronchiolitis obliterans syndrome, Bronchoalveolar lavage fluid, Elastase, Lung, Neutrophils",
author = "Maddalena Cagnone and Davide Piloni and Ilaria Ferrarotti and {Di Venere}, Monica and Simona Viglio and Sara Magni and Anna Bardoni and Roberta Salvini and Marco Fumagalli and Paolo Iadarola and Sabrina Martinello and Federica Meloni",
year = "2019",
month = "3",
day = "1",
doi = "10.3390/ht8010005",
language = "English",
volume = "8",
journal = "High-Throughput",
issn = "2571-5135",
publisher = "MDPI AG",
number = "1",

}

TY - JOUR

T1 - A pilot study to investigate the balance between proteases and α1-antitrypsin in bronchoalveolar lavage fluid of lung transplant recipients

AU - Cagnone, Maddalena

AU - Piloni, Davide

AU - Ferrarotti, Ilaria

AU - Di Venere, Monica

AU - Viglio, Simona

AU - Magni, Sara

AU - Bardoni, Anna

AU - Salvini, Roberta

AU - Fumagalli, Marco

AU - Iadarola, Paolo

AU - Martinello, Sabrina

AU - Meloni, Federica

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count. The results of these experiments demonstrated, for the first time, the presence of the complex between HNE and AAT in a number of BALf samples. The lack of this complex in a few specimens analyzed was investigated in relation to a patient’s lung inflammation. The neutrophil count and the determination of HNE and AAT activities allowed us to speculate that the presence of the complex correlated with the level of lung inflammation.

AB - The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count. The results of these experiments demonstrated, for the first time, the presence of the complex between HNE and AAT in a number of BALf samples. The lack of this complex in a few specimens analyzed was investigated in relation to a patient’s lung inflammation. The neutrophil count and the determination of HNE and AAT activities allowed us to speculate that the presence of the complex correlated with the level of lung inflammation.

KW - Alpha 1-antitrypsin

KW - Bronchiolitis obliterans syndrome

KW - Bronchoalveolar lavage fluid

KW - Elastase

KW - Lung

KW - Neutrophils

UR - http://www.scopus.com/inward/record.url?scp=85064152041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064152041&partnerID=8YFLogxK

U2 - 10.3390/ht8010005

DO - 10.3390/ht8010005

M3 - Article

AN - SCOPUS:85064152041

VL - 8

JO - High-Throughput

JF - High-Throughput

SN - 2571-5135

IS - 1

M1 - 5

ER -