A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking

Pablo S. Aguilar, Florian Fröhlich, Michael Rehman, Mike Shales, Igor Ulitsky, Agustina Olivera-Couto, Hannes Braberg, Ron Shamir, Peter Walter, Matthias Mann, Christer S. Ejsing, Nevan J. Krogan, Tobias C. Walther

Research output: Contribution to journalArticle

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Abstract

The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.

Original languageEnglish
Pages (from-to)901-908
Number of pages8
JournalNature Structural and Molecular Biology
Volume17
Issue number7
DOIs
Publication statusPublished - Jul 2010

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Sphingolipids
Cell Membrane
Genes
Guanine Nucleotide Exchange Factors
Membrane Lipids
Endocytosis
Guanosine Triphosphate
Lipid Metabolism
Blood Proteins
Membrane Proteins

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Aguilar, P. S., Fröhlich, F., Rehman, M., Shales, M., Ulitsky, I., Olivera-Couto, A., ... Walther, T. C. (2010). A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nature Structural and Molecular Biology, 17(7), 901-908. https://doi.org/10.1038/nsmb.1829

A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. / Aguilar, Pablo S.; Fröhlich, Florian; Rehman, Michael; Shales, Mike; Ulitsky, Igor; Olivera-Couto, Agustina; Braberg, Hannes; Shamir, Ron; Walter, Peter; Mann, Matthias; Ejsing, Christer S.; Krogan, Nevan J.; Walther, Tobias C.

In: Nature Structural and Molecular Biology, Vol. 17, No. 7, 07.2010, p. 901-908.

Research output: Contribution to journalArticle

Aguilar, PS, Fröhlich, F, Rehman, M, Shales, M, Ulitsky, I, Olivera-Couto, A, Braberg, H, Shamir, R, Walter, P, Mann, M, Ejsing, CS, Krogan, NJ & Walther, TC 2010, 'A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking', Nature Structural and Molecular Biology, vol. 17, no. 7, pp. 901-908. https://doi.org/10.1038/nsmb.1829
Aguilar, Pablo S. ; Fröhlich, Florian ; Rehman, Michael ; Shales, Mike ; Ulitsky, Igor ; Olivera-Couto, Agustina ; Braberg, Hannes ; Shamir, Ron ; Walter, Peter ; Mann, Matthias ; Ejsing, Christer S. ; Krogan, Nevan J. ; Walther, Tobias C. / A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. In: Nature Structural and Molecular Biology. 2010 ; Vol. 17, No. 7. pp. 901-908.
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