Abstract
The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 901-908 |
| Number of pages | 8 |
| Journal | Nature Structural and Molecular Biology |
| Volume | 17 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2010 |
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ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
Cite this
A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. / Aguilar, Pablo S.; Fröhlich, Florian; Rehman, Michael; Shales, Mike; Ulitsky, Igor; Olivera-Couto, Agustina; Braberg, Hannes; Shamir, Ron; Walter, Peter; Mann, Matthias; Ejsing, Christer S.; Krogan, Nevan J.; Walther, Tobias C.
In: Nature Structural and Molecular Biology, Vol. 17, No. 7, 07.2010, p. 901-908.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking
AU - Aguilar, Pablo S.
AU - Fröhlich, Florian
AU - Rehman, Michael
AU - Shales, Mike
AU - Ulitsky, Igor
AU - Olivera-Couto, Agustina
AU - Braberg, Hannes
AU - Shamir, Ron
AU - Walter, Peter
AU - Mann, Matthias
AU - Ejsing, Christer S.
AU - Krogan, Nevan J.
AU - Walther, Tobias C.
PY - 2010/7
Y1 - 2010/7
N2 - The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
AB - The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
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U2 - 10.1038/nsmb.1829
DO - 10.1038/nsmb.1829
M3 - Article
C2 - 20526336
AN - SCOPUS:77954385031
VL - 17
SP - 901
EP - 908
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 7
ER -