A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge

Antonio Scardino, Maurizio Alimandi, Pierpaolo Correale, Steven G. Smith, Roberto Bei, Hüseyin Firat, Maria Grazia Cusi, Olivier Faure, Stephanie Graf-Dubois, Giulia Cencioni, Jordan Marrocco, Salem Chouaib, François A. Lemonnier, Andrew Michael Jackson, Kostas Kosmatopoulos

Research output: Contribution to journalArticle

Abstract

A cDNA vaccine (pVax1/pet-neu) was designed to encode 12 different Her-2/ErbB-2-derived, HLA-A*0201-restricted dominant and high-affinity heteroclitic cryptic epitopes. Vaccination with pVax1/pet-neu triggered multiple and ErbB-2-specific CTL responses in HLA-A*0201 transgenic HHD mice and in HLA-A*0201 healthy donors in vitro. Human and murine CTL specific for each one of the 12 ErbB-2 peptides recognized in vitro both human and murine tumor cells overexpressing endogenous ErbB-2. Furthermore, vaccination of HHD mice with pVax1/pet-neu significantly delayed the in vivo growth of challenged ErbB-2-expressing tumor (EL4/HHD/neu murine thymoma) more actively when compared with vaccination with the empty vector (pVax1) or vehicle alone. These data indicate that the pVax1/pet-neu cDNA vaccine coding for a poly-ErbB-2 epitope is able to generate simultaneous ErbB-2-specific antitumor responses against dominant and cryptic multiple epitopes.

Original languageEnglish
Pages (from-to)7028-7036
Number of pages9
JournalCancer Research
Volume67
Issue number14
DOIs
Publication statusPublished - Jul 15 2007

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Scardino, A., Alimandi, M., Correale, P., Smith, S. G., Bei, R., Firat, H., Cusi, M. G., Faure, O., Graf-Dubois, S., Cencioni, G., Marrocco, J., Chouaib, S., Lemonnier, F. A., Jackson, A. M., & Kosmatopoulos, K. (2007). A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge. Cancer Research, 67(14), 7028-7036. https://doi.org/10.1158/0008-5472.CAN-06-3998