A proteomic approach for the characterization of C677T mutation of the human gene methylenetetrahydrofolate reductase

Marilena Greco, Fernanda Chiriacò, Piero Del Boccio, Luigi Tagliaferro, Raffaele Acierno, Paola Menegazzi, Eleonore Pinca, Francesco Pignatelli, Carlo Storelli, Giorgio Federici, Andrea Urbani, Michele Maffia

Research output: Contribution to journalArticlepeer-review

Abstract

Methylenetetrahydrofolate reductase (MTHFR) catalyzes the conversion of methylenetetrahydrofolate (CH2H4folate) to methyltetrahydrofolate (CH3H4folate). The C677T mutation is a common polymorphism of the human enzyme that leads to the replacement of Ala222Val, thermolability of MTHFR, and mild elevation of plasma homocysteine levels. A mild hyperhomocysteinemia is known to be risk factor for cardiovascular and thrombotic diseases, ischemic stroke, neural tube defects, late on-set dementia, and pregnancy complications. Human plasma of subjects carrying the C677T mutation in the MTHFR gene has been investigated for their protein pattern in order to identify novel molecular hallmarks. 2-D analysis of the plasma protein allowed the identification of a specific pattern associated with the TT mutant genotype. Noteworthy, we found one spot shifted to a more basic pI in mutant individuals, and MS identification corresponded to vitamin D-binding protein (DBP or group component (Gc) globulin). MS/MS peptide sequencing allowed to discriminate different allelic variants in the investigated clinical groups. These data confirmed by molecular genetic analysis highlight the novel association between the C677T MTHFR genotype with the Gc2 polymorphism of the DBP. Moreover, we found a quantitative reduction of Apolipoprotein A-I in mutant individuals, which was associated, in previous studies by others to an increased cardiovascular risk.

Original languageEnglish
Pages (from-to)5350-5361
Number of pages12
JournalProteomics
Volume6
Issue number19
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Apolipoprotein A-l
  • Cardiovascular diseases
  • Methylenetetrahydrofolate reductase
  • Vitamin D-binding protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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