A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

Chiara Fania; Ilaria Sogno; Michele Vasso; Enrica Torretta; Roberta Leone; Antonino Bruno; Paolo Consonni; Adriana Albini; Cecilia Gelfi

doi:10.1016/j.cca.2014.10.003

A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

Chiara Fania, Ilaria Sogno, Michele Vasso, Enrica Torretta, Roberta Leone, Antonino Bruno, Paolo Consonni, Adriana Albini, Cecilia Gelfi

Research output: Contribution to journal › Article

Abstract

Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value

Original language	English
Pages (from-to)	42-49
Number of pages	8
Journal	Clinica Chimica Acta
Volume	439
DOIs	https://doi.org/10.1016/j.cca.2014.10.003
Publication status	Published - Oct 12 2014

Keywords

Complement C3f
Immunodepletion
MALDI profiling
Prostate cancer
Prostate-specific antigen

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical
Medicine(all)

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Fania, C., Sogno, I., Vasso, M., Torretta, E., Leone, R., Bruno, A., Consonni, P., Albini, A., & Gelfi, C. (2014). A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification. Clinica Chimica Acta, 439, 42-49. https://doi.org/10.1016/j.cca.2014.10.003

A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification. / Fania, Chiara; Sogno, Ilaria; Vasso, Michele; Torretta, Enrica; Leone, Roberta; Bruno, Antonino; Consonni, Paolo; Albini, Adriana ; Gelfi, Cecilia.

In: Clinica Chimica Acta, Vol. 439, 12.10.2014, p. 42-49.

Research output: Contribution to journal › Article

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abstract = "Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value ",

keywords = "Complement C3f, Immunodepletion, MALDI profiling, Prostate cancer, Prostate-specific antigen",

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AU - Fania, Chiara

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AU - Vasso, Michele

AU - Torretta, Enrica

AU - Leone, Roberta

AU - Bruno, Antonino

AU - Consonni, Paolo

AU - Albini, Adriana

AU - Gelfi, Cecilia

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