A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

Chiara Fania; Ilaria Sogno; Michele Vasso; Enrica Torretta; Roberta Leone; Antonino Bruno; Paolo Consonni; Adriana Albini; Cecilia Gelfi

doi:10.1016/j.cca.2014.10.003

A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

Chiara Fania, Ilaria Sogno, Michele Vasso, Enrica Torretta, Roberta Leone, Antonino Bruno, Paolo Consonni, Adriana Albini, Cecilia Gelfi

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value

Original language	English
Pages (from-to)	42-49
Number of pages	8
Journal	Clinica Chimica Acta
Volume	439
DOIs	https://doi.org/10.1016/j.cca.2014.10.003
Publication status	Published - Oct 12 2014

Keywords

Complement C3f
Immunodepletion
MALDI profiling
Prostate cancer
Prostate-specific antigen

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical
Medicine(all)

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10.1016/j.cca.2014.10.003

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title = "A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification",

abstract = "Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value ",

keywords = "Complement C3f, Immunodepletion, MALDI profiling, Prostate cancer, Prostate-specific antigen",

author = "Chiara Fania and Ilaria Sogno and Michele Vasso and Enrica Torretta and Roberta Leone and Antonino Bruno and Paolo Consonni and Adriana Albini and Cecilia Gelfi",

year = "2014",

month = oct,

day = "12",

doi = "10.1016/j.cca.2014.10.003",

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journal = "Clinica Chimica Acta",

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T1 - A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

AU - Fania, Chiara

AU - Sogno, Ilaria

AU - Vasso, Michele

AU - Torretta, Enrica

AU - Leone, Roberta

AU - Bruno, Antonino

AU - Consonni, Paolo

AU - Albini, Adriana

AU - Gelfi, Cecilia

PY - 2014/10/12

Y1 - 2014/10/12

N2 - Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value

AB - Background: Prostate cancer (PCa) is the second cause of mortality in men worldwide. The prostate-specific antigen (PSA) test is routinely adopted in diagnosis; nevertheless more reliable biomarkers are continuously under investigation by monitoring the release of molecules into the bloodstream. The serum protein profiles appear to provide cancer-specific fingerprints that help to discriminate patients (especially with low PSA level) from controls, improving the performance of existing clinical tests. Methods: Samples from healthy controls and PCa patients with low (≤. 4. ng/mL) and high PSA (>. 4. ng/mL) levels were analyzed by MALDI profiling, and by a multi fractionation approach coupled to ESI-MS for peaks identification. Results: MALDI profiling achieved to detect 10 and 14 changed peaks (p-value

KW - Complement C3f

KW - Immunodepletion

KW - MALDI profiling

KW - Prostate cancer

KW - Prostate-specific antigen

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A PSA-guided approach for a better diagnosis of prostatic adenocarcinoma based on MALDI profiling and peptide identification

Abstract

Keywords

ASJC Scopus subject areas

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