A quality control study to assess the inter-laboratory variability of routine estrogen and progesterone receptor assays

David T. Zava, Aenn Wyler-Von Ballmoos, Aron Goldhirsch, Werner Roos, Atsuro Takahashi, Urs Eppenberger, Stefan Arrenbrecht, Georg Martz, Gabriele Losa, Fulgencio Gomez, Christiane Guelpa

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The steroid hormone receptor laboratories of Bern, Basel, Zürich, Locarno, Lausanne and Geneva have participated in a quality control study to assess potential inter-laboratory variability in results of tests for determination of steroid hormone receptor status in human breast tumor biopsies. Homogeneous breast tumor powders containing low, medium and high concentrations of estrogen receptors (ER) and progesterone receptors (PR) were prepared in Bern and dispatched on solid CO2 to each laboratory within 1 day of preparation. Each laboratory was requested to assay each powder for ER and PR by their usual procedures. The results revealed that the quantitative discrepancies in ER and PR binding values among the participants could be attributed in part to variations in the methods used for measuring cytosol protein content and also to the differences in hormone receptor assay methods. Nevertheless, all of the laboratories were able to identify the samples containing low, medium and high concentrations of ER and PR.

Original languageEnglish
Pages (from-to)713-721
Number of pages9
JournalEuropean Journal of Cancer and Clinical Oncology
Volume18
Issue number8
DOIs
Publication statusPublished - 1982

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Progesterone Receptors
Estrogen Receptors
Quality Control
Steroid Receptors
Hormones
Powders
Breast Neoplasms
Cytosol
Biopsy
Proteins

ASJC Scopus subject areas

  • Oncology

Cite this

A quality control study to assess the inter-laboratory variability of routine estrogen and progesterone receptor assays. / Zava, David T.; Wyler-Von Ballmoos, Aenn; Goldhirsch, Aron; Roos, Werner; Takahashi, Atsuro; Eppenberger, Urs; Arrenbrecht, Stefan; Martz, Georg; Losa, Gabriele; Gomez, Fulgencio; Guelpa, Christiane.

In: European Journal of Cancer and Clinical Oncology, Vol. 18, No. 8, 1982, p. 713-721.

Research output: Contribution to journalArticle

Zava, DT, Wyler-Von Ballmoos, A, Goldhirsch, A, Roos, W, Takahashi, A, Eppenberger, U, Arrenbrecht, S, Martz, G, Losa, G, Gomez, F & Guelpa, C 1982, 'A quality control study to assess the inter-laboratory variability of routine estrogen and progesterone receptor assays', European Journal of Cancer and Clinical Oncology, vol. 18, no. 8, pp. 713-721. https://doi.org/10.1016/0277-5379(82)90068-2
Zava, David T. ; Wyler-Von Ballmoos, Aenn ; Goldhirsch, Aron ; Roos, Werner ; Takahashi, Atsuro ; Eppenberger, Urs ; Arrenbrecht, Stefan ; Martz, Georg ; Losa, Gabriele ; Gomez, Fulgencio ; Guelpa, Christiane. / A quality control study to assess the inter-laboratory variability of routine estrogen and progesterone receptor assays. In: European Journal of Cancer and Clinical Oncology. 1982 ; Vol. 18, No. 8. pp. 713-721.
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AU - Roos, Werner

AU - Takahashi, Atsuro

AU - Eppenberger, Urs

AU - Arrenbrecht, Stefan

AU - Martz, Georg

AU - Losa, Gabriele

AU - Gomez, Fulgencio

AU - Guelpa, Christiane

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