A quantitative interpretation of the response of articular cartilage to atomic force microscopy-based dynamic nanoindentation tests

Matteo Taffetani, Roberto Raiteri, Riccardo Gottardi, Dario Gastaldi, Pasquale Vena

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this paper, a quantitative interpretation for atomic force microscopy-based dynamic nanoindentation (AFM-DN) tests on the superficial layers of bovine articular cartilage (AC) is provided. The relevant constitutive parameters of the tissue are estimated by fitting experimental results with a finite element model in the frequency domain. Such model comprises a poroelastic stress-strain relationship for a fibril reinforced tissue constitution, assuming a continuous distribution of the collagen network orientations. The identification procedure was first validated using a simplified transversely isotropic constitutive relationship; then, the experimental data were manually fitted by using the continuous distribution fibril model. Tissue permeability is derived from the maximum value of the phase shift between the input harmonic loading and the harmonic tissue response. Tissue parameters related to the stiffness are obtained from the frequency response of the experimental storage modulus and phase shift. With this procedure, an axial to transverse stiffness ratio (anisotropy ratio) of about 0.15 is estimated.

Original languageEnglish
Article number071005
JournalJournal of Biomechanical Engineering
Volume137
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

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Atomic Force Microscopy
Cartilage
Articular Cartilage
Nanoindentation
Atomic force microscopy
Tissue
Phase shift
Stiffness
Constitution and Bylaws
Anisotropy
Collagen
Frequency response
Permeability
Elastic moduli

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

A quantitative interpretation of the response of articular cartilage to atomic force microscopy-based dynamic nanoindentation tests. / Taffetani, Matteo; Raiteri, Roberto; Gottardi, Riccardo; Gastaldi, Dario; Vena, Pasquale.

In: Journal of Biomechanical Engineering, Vol. 137, No. 7, 071005, 01.07.2015.

Research output: Contribution to journalArticle

Taffetani, Matteo ; Raiteri, Roberto ; Gottardi, Riccardo ; Gastaldi, Dario ; Vena, Pasquale. / A quantitative interpretation of the response of articular cartilage to atomic force microscopy-based dynamic nanoindentation tests. In: Journal of Biomechanical Engineering. 2015 ; Vol. 137, No. 7.
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