A radioimmunoassay for virus antibody using binding of 125I-labelled protein A

A. Colombatti, J. Hilgers

Research output: Contribution to journalArticlepeer-review

Abstract

An assay for virus antibodies using protein A from Staphylococcus aureus is described. Type B and type C RNA tumour viruses adsorbed on to polystyrene microtitre plate wells were incubated with antiserum and then with 125I-labelled protein A (I-pA) and bound radioactivity was determined. Technical details such as labelling, antigen concentration, storage of I-pA are reported. The specificity of the reaction was investigated in detail by competition experiments with purified unbound homologous viruses. This assay also proved to be sensitive for demonstration of autogenous immunity to both type B and type C RNA tumour viruses. A study using antisera against purified care and envelope virus proteins of mammary tumour and leukemia viruses suggested that the reaction mainly involves surface antigens of the intact virions.

Original languageEnglish
Pages (from-to)395-401
Number of pages7
JournalJournal of General Virology
Volume43
Issue number2
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Immunology
  • Virology

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