TY - JOUR
T1 - A rapid liquid chromatography tandem mass spectrometry-based method for measuring propranolol on dried blood spots
AU - Della Bona, Maria Luisa
AU - Malvagia, Sabrina
AU - Villanelli, Fabio
AU - Giocaliere, Elisa
AU - Ombrone, Daniela
AU - Funghini, Silvia
AU - Filippi, Luca
AU - Cavallaro, Giacomo
AU - Bagnoli, Paola
AU - Guerrini, Renzo
AU - La Marca, Giancarlo
PY - 2013/5/5
Y1 - 2013/5/5
N2 - Propranolol, a non-selective beta blocker drug, is used in young infants and newborns for treating several heart diseases; its pharmacokinetics has been extensively evaluated in adult patients using extrapolation to treat pediatric population.The purpose of the present study was to develop and validate a method to measure propranolol levels in dried blood spots. The analysis was performed by using liquid chromatography/tandem mass spectrometry operating in multiple reaction monitoring mode. The calibration curve in matrix was linear in the concentration range of 2.5-200 μg/L with correlation coefficient r=. 0.9996. Intra-day and inter-day precisions and biases were less than 8.0% (n=. 10) and 11.5% (n=. 10) respectively. The recoveries ranged from 94 to 100% and the matrix effect did not result in a severe signal suppression.Propranolol on dried blood spot showed a good stability at three different temperatures for one month.This paper describes a micromethod for measuring propranolol levels on dried blood spot, which determines a great advantage in neonates or young infants during pharmacokinetic studies because of less invasive sampling and small blood volume required.
AB - Propranolol, a non-selective beta blocker drug, is used in young infants and newborns for treating several heart diseases; its pharmacokinetics has been extensively evaluated in adult patients using extrapolation to treat pediatric population.The purpose of the present study was to develop and validate a method to measure propranolol levels in dried blood spots. The analysis was performed by using liquid chromatography/tandem mass spectrometry operating in multiple reaction monitoring mode. The calibration curve in matrix was linear in the concentration range of 2.5-200 μg/L with correlation coefficient r=. 0.9996. Intra-day and inter-day precisions and biases were less than 8.0% (n=. 10) and 11.5% (n=. 10) respectively. The recoveries ranged from 94 to 100% and the matrix effect did not result in a severe signal suppression.Propranolol on dried blood spot showed a good stability at three different temperatures for one month.This paper describes a micromethod for measuring propranolol levels on dried blood spot, which determines a great advantage in neonates or young infants during pharmacokinetic studies because of less invasive sampling and small blood volume required.
KW - Beta-adrenergic receptor blocking agent
KW - Dried blood spot
KW - Liquid chromatography-tandem mass spectrometry
KW - Propranolol
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U2 - 10.1016/j.jpba.2013.01.034
DO - 10.1016/j.jpba.2013.01.034
M3 - Article
C2 - 23434527
AN - SCOPUS:84874518094
VL - 78-79
SP - 34
EP - 38
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -