A rapid liquid chromatography tandem mass spectrometry-based method for measuring propranolol on dried blood spots

Maria Luisa Della Bona, Sabrina Malvagia, Fabio Villanelli, Elisa Giocaliere, Daniela Ombrone, Silvia Funghini, Luca Filippi, Giacomo Cavallaro, Paola Bagnoli, Renzo Guerrini, Giancarlo La Marca

Research output: Contribution to journalArticlepeer-review

Abstract

Propranolol, a non-selective beta blocker drug, is used in young infants and newborns for treating several heart diseases; its pharmacokinetics has been extensively evaluated in adult patients using extrapolation to treat pediatric population.The purpose of the present study was to develop and validate a method to measure propranolol levels in dried blood spots. The analysis was performed by using liquid chromatography/tandem mass spectrometry operating in multiple reaction monitoring mode. The calibration curve in matrix was linear in the concentration range of 2.5-200 μg/L with correlation coefficient r=. 0.9996. Intra-day and inter-day precisions and biases were less than 8.0% (n=. 10) and 11.5% (n=. 10) respectively. The recoveries ranged from 94 to 100% and the matrix effect did not result in a severe signal suppression.Propranolol on dried blood spot showed a good stability at three different temperatures for one month.This paper describes a micromethod for measuring propranolol levels on dried blood spot, which determines a great advantage in neonates or young infants during pharmacokinetic studies because of less invasive sampling and small blood volume required.

Original languageEnglish
Pages (from-to)34-38
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume78-79
DOIs
Publication statusPublished - May 5 2013

Keywords

  • Beta-adrenergic receptor blocking agent
  • Dried blood spot
  • Liquid chromatography-tandem mass spectrometry
  • Propranolol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

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