A rapid method for monitoring DNA labelling reactions with haptens

Angelo Corti, Pasquale Tripputi, Giovanni Cassani

Research output: Contribution to journalArticle

Abstract

A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of Immunological Methods
Volume134
Issue number1
DOIs
Publication statusPublished - Nov 6 1990

Keywords

  • Enzyme immunoassay
  • Hybridisation assay
  • Nonradioactive labelling
  • Nucleic acid

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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