A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans

Sara Epis, Aida Capone, Elena Martin, Michela Paolucci, Chiara Bazzocchi, Matteo Valzano, Jovana Bozic, Stefano Novati, Guido Favia, Irene Ricci

Research output: Contribution to journalArticlepeer-review

Abstract

The yeast Wickerhamomyces anomalus has been proposed for many biotechnological applications in the food industry. However, a number of opportunistic pathogenic strains have been reported as causative agents of nosocomial fungemia. Recognition of potentially pathogenic isolates is an important challenge for the future commercialization of this yeast. The isolation of W. anomalus from different matrices and, recently, from mosquitoes, requires further investigations into its circulation in humans. Here we present a qPCR protocol for the detection of W. anomalus in human blood samples and the results of a screening of 525 donors, including different classes of patients and healthy people.

Original languageEnglish
Pages (from-to)577-581
Number of pages5
JournalNew Microbiologica
Volume38
Issue number4
Publication statusPublished - Oct 1 2015

Keywords

  • Fungemia
  • Mosquito vectors
  • Opportunistic pathogen
  • QPCR
  • Wickerhamomyces anomalus

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans'. Together they form a unique fingerprint.

Cite this