A rapid screening method for routine congener-specific analysis of polychlorinated biphenyls in human serum by high-resolution gas chromatography with mass spectrometric detection

Roberta Turci, Federica Angeleri, Claudio Minoia

Research output: Contribution to journalArticlepeer-review

Abstract

A screening method using gas chromatography/mass spectrometry in the single ion monitoring (SIM) mode for the routine congener-specific analysis of polychlorinated biphenyls (PCBs) in human serum has been developed and validated. A fast procedure incorporating both liquid-liquid and solid-phase extraction was used for clean up and enrichment. Interday and intraday precision and accuracy were assessed for the 12 congeners having dioxin-like activity and PCBs #180 and #170 by analyzing spiked samples on three subsequent days and using 13C12-labelled analogues as internal standards. Furthermore, the relative errors for six non-dioxin-like congeners (PCBs #28, #52, #101, #138, #153 and #180) were measured by using certified reference materials, providing good accuracy at two different concentration levels. The limits of quantification and of detection were 0.5 and 0.2 μg/L in serum for each congener, respectively. The method allows the chromatographic separation of at least 35 congeners and can therefore be applied to the routine monitoring of the general population for both dioxin-like and non-dioxin-like PCB congeners.

Original languageEnglish
Pages (from-to)1957-1964
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume16
Issue number20
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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