A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates

Laura Rindi, Lea Bianchi, Enrico Tortoli, Nicoletta Lari, Daniela Bonanni, Carlo Garzelli

Research output: Contribution to journalArticlepeer-review

Abstract

A real-time PCR genotypic assay was developed for the detection of isoniazid (INH) resistance in Mycobacterium tuberculosis. The assay detects mutations C(-15)T and, possibly, G(-24)T in the regulatory region of the inhA gene and proved as sensitive and specific as nucleotide sequencing in all the clinical isolates tested. Our assays mapped the mutations efficiently in 10 out of 35 resistant isolates, thereby covering 29% of all resistant strains.

Original languageEnglish
Pages (from-to)797-800
Number of pages4
JournalJournal of Microbiological Methods
Volume55
Issue number3
DOIs
Publication statusPublished - Dec 2003

Keywords

  • Isoniazid resistance
  • Mycobacterium tuberculosis
  • Real-time PCR assay

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Fingerprint

Dive into the research topics of 'A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates'. Together they form a unique fingerprint.

Cite this