A recently developed bifacial platelet-rich fibrin matrix

E. Lucarelli, R. Beretta, B. Dozza, P. L. Tazzari, S. M. O'Connell, F. Ricci, M. Pierini, S. Squarzoni, P. P. Pagliaro, E. I. Oprita, D. Donati

Research output: Contribution to journalArticle

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Abstract

Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET®), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 ± 314.6 kPa, stress at a break of 1476.0 ± 526.3 kPa, and an elongation at break of 146.3 ± 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

Original languageEnglish
Pages (from-to)13-23
Number of pages11
JournalEuropean Cells and Materials
Volume20
Publication statusPublished - Jul 2010

Fingerprint

Platelets
Fibrin
Blood Platelets
Mesenchymal Stromal Cells
Mechanical properties
Stem cells
Intercellular Signaling Peptides and Proteins
Platelet-Rich Plasma
Conditioned Culture Medium
Repair
Tissue
Elastic Modulus
Plasmas
Centrifugation
Tears
Epidermal Growth Factor
Thrombin
Cell proliferation
Vascular Endothelial Growth Factor A
Culture Media

Keywords

  • Fibrin
  • Mesenchymal stem cells
  • Physical properties
  • Platelet-rich fibrin matrix (PRFM)
  • Platelet-rich plasma (PRP)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Lucarelli, E., Beretta, R., Dozza, B., Tazzari, P. L., O'Connell, S. M., Ricci, F., ... Donati, D. (2010). A recently developed bifacial platelet-rich fibrin matrix. European Cells and Materials, 20, 13-23.

A recently developed bifacial platelet-rich fibrin matrix. / Lucarelli, E.; Beretta, R.; Dozza, B.; Tazzari, P. L.; O'Connell, S. M.; Ricci, F.; Pierini, M.; Squarzoni, S.; Pagliaro, P. P.; Oprita, E. I.; Donati, D.

In: European Cells and Materials, Vol. 20, 07.2010, p. 13-23.

Research output: Contribution to journalArticle

Lucarelli, E, Beretta, R, Dozza, B, Tazzari, PL, O'Connell, SM, Ricci, F, Pierini, M, Squarzoni, S, Pagliaro, PP, Oprita, EI & Donati, D 2010, 'A recently developed bifacial platelet-rich fibrin matrix', European Cells and Materials, vol. 20, pp. 13-23.
Lucarelli E, Beretta R, Dozza B, Tazzari PL, O'Connell SM, Ricci F et al. A recently developed bifacial platelet-rich fibrin matrix. European Cells and Materials. 2010 Jul;20:13-23.
Lucarelli, E. ; Beretta, R. ; Dozza, B. ; Tazzari, P. L. ; O'Connell, S. M. ; Ricci, F. ; Pierini, M. ; Squarzoni, S. ; Pagliaro, P. P. ; Oprita, E. I. ; Donati, D. / A recently developed bifacial platelet-rich fibrin matrix. In: European Cells and Materials. 2010 ; Vol. 20. pp. 13-23.
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