A receptor-antibody sandwich assay for teicoplanin

A. Corti, L. Cavenaghi, E. Giani, G. Cassani

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a new method for quantifying teicoplanin in complex matrixes, a receptor-antibody sandwich assay (RASA). The method is based on bioselective adsorption of teicoplanin onto microtiter plates coated with albumin-ε-aminocaproyl-D-alanyl-D-alanine, a synthetic analog of its biological target, and reaction with anti-teicoplanin antibodies. The sandwich complexes are detected by incubation with peroxidase-labeled goat antibodies to rabbit IgGs and chromogenic reaction with o-phenylenediamine. The dose-response curve was linear for teicoplanin concentrations in the range from 0 to 0.15 mg/L. We used the assay to measure teicoplanin concentrations in various biological matrixes. Analytical recovery from serum was 99.5%, the interassay CV was 5.1%, and the detection limit was 30 μg/L (P

Original languageEnglish
Pages (from-to)1615-1618
Number of pages4
JournalClinical Chemistry
Volume33
Issue number9
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Clinical Biochemistry

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