Abstract
In order to investigate whether Glut 4 content is reduced in the adipose tissue at an early stage of human insulin resistance, among 30 non-obese (BMI <27) non-diabetic (by oral glucose tolerance test) volunteers, we selected 3 groups of 10 subjects each according to their insulin sensitivity at an i.v. insulin tolerance test (K(itt) values). We then compared the Glut 4 content in the adipose tissue specimens from 7 of the most insulin sensitive and 8 of the most insulin resistant subjects. No difference was observed in the average Glut 4 content between the 2 groups (0.45 ± 0.05 arbitrary densitometric units in insulin sensitive subjects vs 0.61 ± 0.16 in insulin-resistant subjects). No significant correlation was observed between Glut 4 content in adipose tissue nor in vivo insulin sensitivity (K(itt) values), plasma insulin levels 120 min after an oral glucose load, or BMI. In contrast to Glut 4 content, insulin action was already reduced in adipose tissue from insulin-resistant subjects, as shown by the significantly (p <0.05) impaired ability to stimulate receptor tyrosine-kinase activity, a key step in the insulin signalling cascade. In conclusion we demonstrate that, at an early stage, human insulin resistance is not associated to a reduced Glut 4 content in adipose tissue.
| Original language | English |
|---|---|
| Pages (from-to) | 262-266 |
| Number of pages | 5 |
| Journal | Diabetes, Nutrition and Metabolism - Clinical and Experimental |
| Volume | 8 |
| Issue number | 5 |
| Publication status | Published - 1995 |
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Keywords
- Andipose tissue
- Glucose transport
- Insulin receptor
- Tyrosine-kinase
ASJC Scopus subject areas
- Food Science
- Endocrinology
- Medicine (miscellaneous)
- Endocrinology, Diabetes and Metabolism
- Internal Medicine
Cite this
A reduced Glut 4 content in adipose tissue is not an early defect in the development of human insulin resistance. / Frittitta, L.; Grasso, G.; Vigneri, R.; Trischitta, V.
In: Diabetes, Nutrition and Metabolism - Clinical and Experimental, Vol. 8, No. 5, 1995, p. 262-266.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A reduced Glut 4 content in adipose tissue is not an early defect in the development of human insulin resistance
AU - Frittitta, L.
AU - Grasso, G.
AU - Vigneri, R.
AU - Trischitta, V.
PY - 1995
Y1 - 1995
N2 - In order to investigate whether Glut 4 content is reduced in the adipose tissue at an early stage of human insulin resistance, among 30 non-obese (BMI <27) non-diabetic (by oral glucose tolerance test) volunteers, we selected 3 groups of 10 subjects each according to their insulin sensitivity at an i.v. insulin tolerance test (K(itt) values). We then compared the Glut 4 content in the adipose tissue specimens from 7 of the most insulin sensitive and 8 of the most insulin resistant subjects. No difference was observed in the average Glut 4 content between the 2 groups (0.45 ± 0.05 arbitrary densitometric units in insulin sensitive subjects vs 0.61 ± 0.16 in insulin-resistant subjects). No significant correlation was observed between Glut 4 content in adipose tissue nor in vivo insulin sensitivity (K(itt) values), plasma insulin levels 120 min after an oral glucose load, or BMI. In contrast to Glut 4 content, insulin action was already reduced in adipose tissue from insulin-resistant subjects, as shown by the significantly (p <0.05) impaired ability to stimulate receptor tyrosine-kinase activity, a key step in the insulin signalling cascade. In conclusion we demonstrate that, at an early stage, human insulin resistance is not associated to a reduced Glut 4 content in adipose tissue.
AB - In order to investigate whether Glut 4 content is reduced in the adipose tissue at an early stage of human insulin resistance, among 30 non-obese (BMI <27) non-diabetic (by oral glucose tolerance test) volunteers, we selected 3 groups of 10 subjects each according to their insulin sensitivity at an i.v. insulin tolerance test (K(itt) values). We then compared the Glut 4 content in the adipose tissue specimens from 7 of the most insulin sensitive and 8 of the most insulin resistant subjects. No difference was observed in the average Glut 4 content between the 2 groups (0.45 ± 0.05 arbitrary densitometric units in insulin sensitive subjects vs 0.61 ± 0.16 in insulin-resistant subjects). No significant correlation was observed between Glut 4 content in adipose tissue nor in vivo insulin sensitivity (K(itt) values), plasma insulin levels 120 min after an oral glucose load, or BMI. In contrast to Glut 4 content, insulin action was already reduced in adipose tissue from insulin-resistant subjects, as shown by the significantly (p <0.05) impaired ability to stimulate receptor tyrosine-kinase activity, a key step in the insulin signalling cascade. In conclusion we demonstrate that, at an early stage, human insulin resistance is not associated to a reduced Glut 4 content in adipose tissue.
KW - Andipose tissue
KW - Glucose transport
KW - Insulin receptor
KW - Tyrosine-kinase
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M3 - Article
AN - SCOPUS:0029586703
VL - 8
SP - 262
EP - 266
JO - Diabetes, Nutrition and Metabolism - Clinical and Experimental
JF - Diabetes, Nutrition and Metabolism - Clinical and Experimental
SN - 0394-3402
IS - 5
ER -