A regulated secretory pathway in cultured hippocampal astrocytes

Federico Calegari, Silvia Coco, Elena Taverna, Monique Bassetti, Claudia Verderio, Nicoletta Corradi, Michela Matteoli, Patrizia Rosa

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Glial cells have been reported to express molecules originally discovered in neuronal and neuroendocrine cells, such as neuropeptides, neuropeptide processing enzymes, and ionic channels. To verify whether astrocytes may have regulated secretory vesicles, the primary cultures prepared from hippocampi of embryonic and neonatal rats were used to investigate the subcellular localization and secretory pathway followed by secretogranin II, a well known marker for dense-core granules. By indirect immunofluorescence, SgII was detected in a large number of cultured hippocampal astrocytes. Immunoreactivity for the granin was detected in the Golgi complex and in a population of dense-core vesicles stored in the cells. Subcellular fractionation experiments revealed that SgII was stored in a vesicle population with a density identical to that of the dense-core secretory granules present in rat pheochromocytoma cells. In line with these data, biochemical results indicated that 40-50% of secretogranin H synthesized during 18-h labeling was retained intracellularly over a 4-h chase period and released after treatment with different secretagogues. The most effective stimulus appeared to be phorbol ester in combination with ionomycin in the presence of extracellular Ca2+, a treatment that was found to produce a large and sustained increase in intracellular calcium [Ca2+](i) transients. Our findings indicate that a regulated secretory pathway characterized by (i) the expression and stimulated exocytosis of a typical marker for regulated secretory granules, (ii) the presence of dense- core vesicles, and (iii) the ability to undergo [Ca2+](i) increase upon specific stimuli is present in cultured hippocampal astrocytes.

Original languageEnglish
Pages (from-to)22539-22547
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number32
DOIs
Publication statusPublished - Aug 6 1999

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Secretory Pathway
Secretory Vesicles
Astrocytes
Chromogranins
Neuropeptides
Rats
Secretogranin II
Ionomycin
Phorbol Esters
Fractionation
Ion Channels
Labeling
Neuroendocrine Cells
Calcium
Exocytosis
Golgi Apparatus
Pheochromocytoma
Indirect Fluorescent Antibody Technique
Molecules
Neuroglia

ASJC Scopus subject areas

  • Biochemistry

Cite this

Calegari, F., Coco, S., Taverna, E., Bassetti, M., Verderio, C., Corradi, N., ... Rosa, P. (1999). A regulated secretory pathway in cultured hippocampal astrocytes. Journal of Biological Chemistry, 274(32), 22539-22547. https://doi.org/10.1074/jbc.274.32.22539

A regulated secretory pathway in cultured hippocampal astrocytes. / Calegari, Federico; Coco, Silvia; Taverna, Elena; Bassetti, Monique; Verderio, Claudia; Corradi, Nicoletta; Matteoli, Michela; Rosa, Patrizia.

In: Journal of Biological Chemistry, Vol. 274, No. 32, 06.08.1999, p. 22539-22547.

Research output: Contribution to journalArticle

Calegari, F, Coco, S, Taverna, E, Bassetti, M, Verderio, C, Corradi, N, Matteoli, M & Rosa, P 1999, 'A regulated secretory pathway in cultured hippocampal astrocytes', Journal of Biological Chemistry, vol. 274, no. 32, pp. 22539-22547. https://doi.org/10.1074/jbc.274.32.22539
Calegari, Federico ; Coco, Silvia ; Taverna, Elena ; Bassetti, Monique ; Verderio, Claudia ; Corradi, Nicoletta ; Matteoli, Michela ; Rosa, Patrizia. / A regulated secretory pathway in cultured hippocampal astrocytes. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 32. pp. 22539-22547.
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