A relationship between replicon size and supercoiled loop domains in the eukaryotic genome

Mario Buongiorno-Nardelli, Gioacchino Micheli, Maria Teresa Carrĩ, Monique Marilley

Research output: Contribution to journalArticle

Abstract

In interphase nuclei and metaphase chromosomes, eukaryotic chromosomal DNA is arranged in negatively supercoiled loops whose length averages several tens of kilobases (kb). Supercoiling indicates that the loops are tenaciously bound at both ends through the periodic attachment of DNA to a non-histone protein structural support, termed the nuclear matrix, skeleton or cage in interphase nuclei and the scaffold in metaphase chromosomes1-16. The looped organization has been envisaged to be important in DNA replication 2,11,15,17-25 and transcription26-28. We report here a close relationship between average loop length and replicon size in different animal and plant species. Autoradiographic experiments support the hypothesis that DNA synthesis occurs at fixed sites on the nuclear matrix15,20. We also describe a modification of the looped organization occurring during early embryonic development in Xenopus laevis.

Original languageEnglish
Pages (from-to)100-102
Number of pages3
JournalNature
Volume298
Issue number5869
DOIs
Publication statusPublished - 1982

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Replicon
Interphase
Genome
Metaphase
DNA
Nuclear Matrix
Xenopus laevis
DNA Replication
Skeleton
Embryonic Development
Chromosomes
Proteins

ASJC Scopus subject areas

  • General

Cite this

A relationship between replicon size and supercoiled loop domains in the eukaryotic genome. / Buongiorno-Nardelli, Mario; Micheli, Gioacchino; Carrĩ, Maria Teresa; Marilley, Monique.

In: Nature, Vol. 298, No. 5869, 1982, p. 100-102.

Research output: Contribution to journalArticle

Buongiorno-Nardelli, M, Micheli, G, Carrĩ, MT & Marilley, M 1982, 'A relationship between replicon size and supercoiled loop domains in the eukaryotic genome', Nature, vol. 298, no. 5869, pp. 100-102. https://doi.org/10.1038/298100a0
Buongiorno-Nardelli, Mario ; Micheli, Gioacchino ; Carrĩ, Maria Teresa ; Marilley, Monique. / A relationship between replicon size and supercoiled loop domains in the eukaryotic genome. In: Nature. 1982 ; Vol. 298, No. 5869. pp. 100-102.
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